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TABLE OF CONTENTS:
- Tomato BIBAC
Libraries:
- Two tomato genomic BIBAC libraries were constructed as part
of a collaboration with Dr. Hong-Bin Zhang at the Texas A&M BAC
center.
- These libraries are described in a Plant Journal article
Hamilton et al., 17 (1999), Plant J 17 (1999), 223-229.
- After publication, these libraries will be available, at
cost, for research purposes only, from the Texas A&M BAC Center.
- We believe that a sole source for the storage and replication
of these libraries will ensure that their quality will not be
compromised.
- Please contact Dr. Hongbin Zhang or Chantel Scheuring at
the "tamu" BAC center
for more information.
- Licensing options are also available from Texas A&M/Cornell
University.
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- BIBAC Standard
Kit Components:
- BIBAC Materials Kit
Revised March 1998
Strains: Escherichia coli (plasmids
all in DH10B)
BIBAC2
pCH20
pCH30 (VirG)
pCH32 (VirGVirE1/VirE2)
All as stabs in LB
+ 40 mg/L kanamycin (BIBAC2, pCH20)
+ 10 mg/L tetracycline (pCH30, pCH32)
Strains: Agrobacterium tumefaciens
| COR314 |
UIA143 pMP90 pCH32 BIBAC2 |
| COR356 |
UIA143 pMP90 pCH32 BIBAC2.H150 |
stabs, LB+ 50 mg/L kanamycin + 5 mg/L tetracycline
COR366 LBA4404 pCH32 BIBAC2
stab, LB + 50 mg/L kanamycin + 2 mg/L tetracycline
COR308 UIA143 pMP90 pCH32
stab, LB + 5 mg/L tetracycline
Documentation
Biological Materials Transfer Agreement _____
Maps: BIBAC2, pCH20, pCH30, pCH32 _____
Notes
Liquid cultures of A. tumefaciens strains, should have
antibiotics added at the Concentrations indicated for the stabs.
In particular, pCH30 and pCH32 are not stable in the absence
of selection (tetracycline).
To select for inserts in the sacB gene we plate out
E. coli cells on LB + 40 mg/L kanamycin + 5% sucrose.
[For library preparation we use LB + 80 mg/L kanamycin, to reduce
a common contaminant in commercially available DH10B ElectroMAX
competent cells (BRL).]
The sacB gene also works in Agrobacterium. So
Agrobacterium strains that contain the BIBAC vector without
insert will be sensitive to high levels of sucrose. Check your
co-cultivation medium; if it contains high levels of sucrose,
then the Agrobacterium cells will be killed. We substituted
glucose with no problems. This is not a problem for BIBAC + insert,
since the sacB gene has been inactivated.
You can use your standard plant transformation protocol. The
only modification that we made (mentioned in the PNAS paper)
was to increase the concentration of the Agrobacterium
cells/ml that was used to dip the tobacco leaf strips. This did
improve efficiency. We are currently testing this modification
for tomato transformation.
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- Licensing
Information:
- Transfer of BIBAC materials is overseen by the Cornell Research
Foundation. CRF requires that a "for research purposes only"
Materials Transfer Agreement be signed before BIBAC materials
can be made available to industrial or academic laboratories.
To obtain materials "for research purposes only" please
contact Maureen Hanson at mrh5@cornell.edu. Provision of these materials requires a $100 handling fee, payable to Cornell University.
BIBAC technology and related materials are available for licensing.
The "intellectual property" that is the basis of BIBAC
technology is the "backbone" of the binary-BAC plasmid.
The "backbone" plasmid (pCH20)
does not contain any plant selectable markers. CRF expects that
(most) potential licensees will want to introduce proprietary
markers. The BIBAC vector was designed with this in mind, and
can be easily modified to specifications. To inquire about licensing,
interested parties may contact Alice Li, xl11@cornell.edu
for additional information.
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- Regarding MOG101
Strains:
- Permission to use Agrobacterium tumefaciens strain
MOG101
Contact Information
- Please contact either of these individuals at Syngenta to obtain the Mogen strains:
Jan Melchers
jan.melchers@syngenta.com
Anja Dol
anja.dol@syngenta.com
Once you have permission from Syngenta to use MOG101 (this strain was formerly owned by MOGEN International), then all of the strains derived from MOG101 by Carol Hamilton will be available for your use.
MOG101 derived materials
Derivatives of MOG101 [C58 pMOG101]
UIA143: recA- deletion of C58
| chromosomal |
pTi |
vir helper |
BIBAC |
COR# |
| |
|
|
|
|
| UIA143 |
pMOG101 |
|
|
|
| UIA143 |
pMOG101 |
pCH30 |
|
COR303 |
| UIA143 |
pMOG101 |
pCH32 |
|
COR309 |
| UIA143 |
pMOG101 |
pCH30 |
BIBAC2 |
COR307 |
| UIA143 |
pMOG101 |
pCH30 |
BIBAC2.H15 |
COR326 |
| UIA143 |
pMOG101 |
pCH32 |
BIBAC2 |
COR316 |
| UIA143 |
pMOG101 |
pCH32 |
BIBAC2.H150 |
COR320 |
| UIA143 pMOG101 pCH* |
LB + Tet5 |
| UIA143 pMOG101 pCH* BIBAC* |
LB + Kan50 + Tet5 |
recA + (MOG101) derivatives available by request
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- How to obtain
BIBAC materials:
- If you are interested in obtaining BIBAC materials for research
purposes, please contact Dr. Maureen Hanson (mrh5@cornell.edu)
at the Plant Science Center, Cornell University. You will be
sent two copies of a Materials Transfer Agreement (MTA) provided
by Cornell Research Foundation (CRF). Once the two copies have
been returned with your signature, a standard BIBAC kit will
be sent to you. If you require something other than the standard
kit, please state that clearly. One copy of the MTA will be returned
to you with the kit and the other retained by CRF for their records.
If you are interested in licensing BIBAC technology, then
please contact Alice Li, xl11@cornell.edu,
(607) 257-1081, at the Cornell Center for Technology, Enterprise & Commercialization (CCTEC), 20 Thornwood Drive, Suite 105, Ithaca, NY 14850.
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