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Genotyping by sequencing (GBS) enables the analysis of large numbers of single nucleotide polymorphisms (SNPs) in studies of genetic variation in a diverse range of species. GBS uses a highly multiplexed, cost effective approach that includes the use of restriction enzymes to reduce genome complexity and next generation sequencing technologies for SNP analysis. We offer GBS project design and optimization services, including on enzyme choice, multiplexing level, and SNP calling procedures. Click here for more information.
We accept 96-well plates of DNA samples for GBS. Highly multiplexed GBS libraries are constructed using restriction enzymes. Click here for more information.
GBS libraries are sequenced on the Illumina HiSeq 2000/2500 (100 bp, single-end reads). Click here for more information.
SNP analysis of GBS data is done by the facility and the results are provided to the user. Click here for more information.
Consultation services are available upon request. Coordinated project design consultation and data analysis support are available with all the other BRC cores. Click here for more information.
The facility offers GBS workshops that include both lectures and hands-on data analysis sessions. Click here for more information.