Library Construction

Standard Library Prep

10X Single Cell RNA-Seq

10X Genomic Linked Reads

High-throughput, full service Whole Genome Sequencing (WGS)

High-throughput, full service 3' RNASeq

High-throughput, full service Custom Genotyping (rAmpSeq)

Sanger Sequencing and Fragment Analysis samples

 

DNA sequencing library sample preparation 

Sequencing libraries can be constructed from purified DNA, purified total RNA, or purified polyA-selected RNA. Illumina sequencing libraries are constructed with Illumina TruSeq protocols for DNA-seq, RNA-seq, and ChIP-seq samples. Illumina Nextera library preps may be used for DNA-seq samples when the total amount of DNA is limiting.

Link to Illumina Library Prep Sample Submission amounts

 Barcoding of purified PCR products for multiplex sequencing of custom amplicons is now available using either Nextera or TruSeq dual indices. (Inquire: BRC_Genomics@cornell.edu).


 10X Genomics library preparation services are available for both single-cell RNA-Seq library construction and genomic DNA library construction for their linked-read technology.

Link to 10X Genomics Chromium Library Prep Information (pending)

 


 High-throughput, full service Whole Genome Sequencing (WGS)

Library construction, including sample quantification and library QC, is currently available for TruSeq-equivalent libraries (8 sample minimum), standard Nextera or Nextera XT libraries (8 sample minimum), or low cost, 1/3 concentration Nextera skim sequencing libraries (95 sample minimum).

Sample submission. Inquire: BRC_genomics-projects@cornell.edu

 


 High-throughput, full service 3’RNAseq

The Genomic Diversity Facility now offers high-throughput 3’ RNA seq (48- or 96-plex) using the Lexogen Quantseq FWD kit.

3’RNA seq is the best alternative to microarrays and conventional RNA seq for gene counting, expression and eQTL studies. High strand specificity allows discovery and quantification of antisense transcripts and overlapping genes.  Because the exact position of the 3’end of the polyA RNA is determined, accurate sequence information is obtained for the 3’UTR.  More reliable gene expression values are obtained because only one cDNA is produced per transcript (length normalization is not required).  Unlike conventional RNA seq, however, 3’RNA seq does not generate information on splice-site junctions/alternative splicing.

Sample submission. Inquire: BRC_Genomics-projects@cornell.edu

 


 High-throughput, full service Custom Genotyping

A new genotyping method, rAmpSeq, based on amplifying unique loci (several thousand per DNA sample) using primers anchored in repetitive DNA (i.e., transposable elements) has been developed for maize (Zea mays), cassava (Manihot esculenta), and sorghum (Sorghum bicolor). Because up to 3,072 samples can be multiplexed in one sequencing lane, this technology is well-suited for large genomic selection projects. (Inquire: BRC_genomics-projects@cornell.edu).

 


 Sanger Sequencing and Fragment Analysis samples

Sample preparations (DNA/RNA isolation and/or PCR amplification) for Full Service DNA sequencing, Fragment Analysis plates, Ready-To-Load DNA sequencing plates, and RT-PCR analysis are not available in the Genomics Facility, and are the responsibility of the user.