Price List

 

Proteomics and Mass Spectrometry Services and Fees for FY15-16

 

Service Code unit of service   Internal price External price
10- Sample Preparation      
1001 Sample Prep Optimization (non SOP) per hour $60.00 $96.00
1002 Bradford Assay set up $40.00 $64.00
1003 Bradford Assay per sample $5.00 $8.00
1004 Affinity depletion of serum high abundant proteins Per sample $100.00 $160.00
1005 iTRAQ 4-plex  reagent per set of samples $280.00 $448.00
1005a iTRAQ 4-plex labeling and sample preparation per set of samples $500.00 $800.00
1005b iTRAQ 8-plex reagent per set of samples $560.00 $896.00
1005c iTRAQ 8-plex labeling and sample preparation per set of samples $700.00 $1,120.00
1006 TMT 6-plex reagent per set of samples $380.00 $608.00
1006a TMT 6-plex labeling and sample preparation per set of samples $600.00 $960.00
1006b TMT10-plex reagent per set of samples $750.00 $1,200.00
1006c TMT 10-plex labeling and sample preparation per set of samples $800.00 $1,280.00
1007 Buffer exchange by ultrafiltration/dialysis/spin column per sample $25.00 $40.00
1008 Spin filtration per sample $15.00 $24.00
1009 Spin cartridge desalt per sample $25.00 $40.00
1010 Gel-based protein quantitation for shotgun per set of samples $200.00 $320.00
1011 dimethylation duplex labeling per set of samples $400.00 $640.00
1012 dimethylation triplex labeling per set of samples $500.00 $800.00
1101 mini SDS-PAGE (precast) per gel $85.00 $136.00
1102 mid SDS-PAGE or native gel (in-house made) per gel $135.00 $216.00
1103 2DE - base Charge base charge set up $250.00 $400.00
1103a 2DE - Base Charge additional gel (inc CCB stain) base charge per gel $200.00 $320.00
1104 mini 2DE - base Charge base charge set up $150.00 $240.00
1104a mini 2DE - Base Charge additional gel (inc CCB stain) base charge per gel $70.00 $112.00
1105 2DE - DIGE Labelling per gel $100.00 $160.00
1106 2DE - Sypro Ruby stain or Pro-Q Diamond per gel $75.00 $120.00
1107 Gel transfer to PVDF for Western blot per gel $100.00 $160.00
1201 Gel Imaging per hour $60.00 $96.00
1202 Image Analysis of 2D gel for top 10 differential spots  per gel $270.00 $432.00
1203 ImageQuant analysis for 1D gel bands per gel $50.00 $80.00
1301 Spot Picking (per spot manual/robotic) per spot $5.00 $8.00
1302 In-gel Digest/Extraction (Robotic) per sample $25.00 $40.00
1303 In-gel Digest/Extraction (Robotic) per plate $2,000.00 $3,200.00
1304 In-gel Digest/Extraction (Manual) set up $100.00 $160.00
1305 In-gel Digest/Extraction (Manual) per sample $50.00 $80.00
1306 In-solution digestion set up $100.00 $160.00
1307 In-solution digestion per sample $50.00 $80.00
1308 second enzyme digestion per sample $25.00 $40.00
1401 ZipTip Cleanup (Robotic) per sample $8.00 $12.80
1402 ZipTip (Manual) per sample $15.00 $24.00
1403 SPE Cleanup (Manual) per sample $25.00 $40.00
1404 SCX Cartridge (Manual) Per sample $40.00 $64.00
1405 PGC Cartirdge (Manual) Per sample $40.00 $64.00
1406 Beads-based IMAC or TiO2 enrichment Per sample $60.00 $96.00
1407 MCX Cartridge (manual) Per sample $60.00 $96.00
         
         
20- HPLC and Mass Spectrometry Analysis      
2001 Ion exchange chromatography with fractions collection per sample $250.00  
2002 high pH reverse phase with fractions collection per sample $250.00 $400.00
2003 HILIC chromtography with fractions collection per sample $250.00 $400.00
2004 Reverse phase chromatography no fractions collection per sample $120.00 $192.00
2005 HPLC method development per sample/analyte $220.00 $352.00
2201 Infusion ESI-MS for small molecule ID per sample $65.00 $104.00
2202 Infusion ESI-MS/MS for addition ions per ion $15.00 $24.00
2203 Infusion ESI-MS/MS for protein IDs and PTMs per sample $100.00 $160.00
2204 Infusion ESI-MS for intact protein MW per sample $80.00 $128.00
2205 nanoInfusion ESI-MS analysis set up $100.00 $160.00
2301 microLC-MS/MS (15min gradient) for rapid protein IDs per sample (1-10 samples) $85.00 $136.00
2302 microLC-MS/MS (15min gradient) for rapid protein IDs per sample (>10 samples) $70.00 $112.00
2303 nanoLC-MS/MS: 15min gradient per sample (1-10 samples) $100.00 $160.00
2304 nanoLC-MS/MS: 15min graident per sample (>10 samples) $85.00 $136.00
2305 nanoLC-MS/MS: 30min gradient per sample $125.00 $200.00
2306 nanoLC-MS/MS: 60min gradient per sample $160.00 $256.00
2306a nanoLC-MS/MS: 60min gradient in Orbitrap Fusion per sample $200.00 $320.00
2307 nanoLC-MS/MS: 90-120min gradient per sample $200.00 $320.00
2307a nanoLC-MS/MS: 90-120min gradient in Orbitrap Fusion per sample $250.00 $400.00
2308 nanoLC-MS/MS: 240min gradient per sample $320.00 $512.00
2401 LC/MS/MS for protein PTMs in two runs (CID and HCD) per sample $250.00 $400.00
2402 LC/MS/MS for iTRAQ-based protein quan/ID in duplicate run per fraction $240.00 $384.00
2403 Additional exclusion list-based LC-MS/MS run for protein IDs per sample $160.00 $256.00
2404 Additional Hypothesis-driven LC-MS/MS run for PTM discovery per sample $120.00 $192.00
2405 MRM-LC-MS/MS for peptide quantitation 30min gradient per sample $120.00 $192.00
2406 MRM-LC-MS/MS for peptide quantitation->=60min gradient per sample $200.00 $320.00
2501 Small molecule quantitation setup/method dev. per analyte $1,200.00 $1,920.00
2502 Small molecule quantitation per sample (<50 samples) $125.00 $200.00
2503 Small molecule quantitation per sample (>=50 samples) $90.00 $144.00
2601 online desalting intact protein MW set up $150.00 $240.00
2602 online desalting intact protein MW per sample $120.00 $192.00
2603 Relative quantitation by label-free nanoLC-MS/MS  per sample $300.00 $480.00
         
         
30- HPLC and MS Data Interpretation      
3001 Peptide quantitation by extracted ion chromatogram per hour $100.00 $160.00
3002 MS/MS Manual Data Interpretation per hour for all relevant spectra $100.00 $160.00
3003 De Novo Sequencing Data Analysis per peptide sequence $100.00 $160.00
3004 Quantitation analysis for label-free nanoLC-MS/MS  per hour $100.00 $160.00
3005 Customized database interrogation  per database $75.00 $120.00
3006 Database search training per hour $60.00 $96.00
3007 Database search aginst an additional database per hour $60.00 $96.00
3008 LC/MS spectra slides for presentation per hour $100.00 $160.00
3009 Additional database search and report per hour $100.00 $160.00
3010 Lebel-free quantitation analysis per hour $100.00 $160.00
         
         
40- Instrument/Software Use Charge      
4001 4000 Q Trap training per hour $150.00 $240.00
4002 Typhoon Training per hour $75.00 $120.00
4003 Typhoon 9400 Access per hour $60.00 $96.00
4004 2DE - Equipment Access per day $100.00 $160.00
4006 ProPrep Access per day $125.00 $200.00
4007 Agilent 1100 HPLC Access per day $125.00 $200.00
4008 U3000 Access per half day (<4 hours) $120.00 $192.00
4009 U3000 Access per day (<12 hours) $180.00 $288.00
4010 U3000 Access per day (24 hours) $200.00 $320.00
4011 4000 QTRAP Access per half day (<4 hours) $300.00 $480.00
4012 4000 QTRAP Access per day (<12 hours) $500.00 $800.00
4013 4000 QTRAP Access per day (24 hours) $600.00 $960.00
4014 LTQ Orbitrap Elite Access per day (<12 hours) $650.00 $1,040.00
4015 LTQ Orbitrap Elite Access per day $800.00 $1,280.00
4016 Gel Image Analysis - Software Access per hour $15.00 $24.00
4020 U3000 nanoRSLC Access per half day (<4 hours) $120.00 $192.00
4021 U3000 nanoRSLC Access per day (<12 hours) $180.00 $288.00
4022 U3000 nanoRSLC Access per day (24 hours) $200.00 $320.00
4023 SpectraMax Plate Reader Access per hour $35.00 $56.00