LC-MS/nanoLC-MS systems

UltiMate 3000 RSLCnano / Orbitrap Fusion

The UltiMate3000 RSLCnano /Orbitrap Fusion Mass Spectrometer (Thermo Scientific) provides both nano and analytical scale of high mass accuracy, ultra high resolution and fast-scanning LC-MS/MS analyses for a broad range of applications, from in-depth discovery experiments to characterization of complex PTMs to comprehensive qualitative/quantitative workflows. The Tribrid architecture (quadrupole, Orbitrap and ion trap mass analyzers) with the availability of multiple fragmentation techniques (CID, HCD, ETD and EThCD) at any stage of MSn is especially powerful when facing complex and low abundance samples in proteomics, glycomics, lipidomics and metabolomics. 


UltiMate 3000 RSLCnano / Orbitrap Elite

 

UltiMate 3000 RSLCnano / LTQ Orbitrap Velos

The Orbitrap Elite Hybrid Mass Spectrometer (Thermo Scientific) provides on-line LC-MS/MS for data-dependent protein IDs and shotgun-based quantitative proteomics using either: (1) in parallel, the high resolution Orbitrap mass analyzer for MS survey scans and the dual-pressure linear ion trap (Velos Pro) for CID-based MS/MS scans; or (2) the Orbitrap mass analyzer for both MS survey and MS/MS scans under HCD mode or with data dependent parallel multiple-stage fragmentation (MSn) analysis.  The high mass accuracy (<3 ppm RMS with external calibration) and high resolution (up to 240,000) offered by the Oribitrap mass analyzer allows for high confidence results with PTM analysis, de novo sequencing and small molecule identification.

The ability of the UltiMate 3000 RSLCnano (Thermo/Dionex) to operate at high pressure (up to 800 bars) with direct nano flow, enables nano-scale separations with this instrument to attain high sensitivity, resolution and reproducibility for complex protein/peptide samples in shotgun proteomics.  The system also provides analytical scale HPLC analysis at high flow rates coupled with the high resolution and high mass accuracy Orbitrap mass analyzer, for small molecule profiling studies and for on-line desalting of intact protein analysis. 

The CorConneX nanospray ion source (CorSolution) utilizes interchangeable column cartridges and low dead-volume compression connections. It is coupled to the Ultimate 3000 RSLCnano and mounted to the LTQ Orbitrap Velos.  This setup facilitates high sensitivity analysis and provides flexible switching of different columns and an infusion analysis mode.

 


UltiMate3000 2DLC / 4000 Q Trap

UltiMate3000 2DLC - 4000 Q Trap

The 4000 Q Trap (ABSciex) features a hybrid triple quadrupole linear ion trap that enables operation in triple quadrupole mode or linear ion trap mode separately or a combination of both, which allows precursor ion scan-triggered MS/MS, neutral loss-triggered MS/MS and MRM-triggered MS/MS with data dependent acquisition other than the regular EMS-triggered MS/MS for high sensitivity protein IDs.  Thee resulting enhanced selective detection can be particularly valuable for hypothesis-driven PTM discovery analysis and target-based validation/confirmation analysis of low abundance analytes in complex samples.

The UltiMate3000 MDLC (Thermo/Dionex) has a built-in micro fraction collection option that allows for automated off-line 2D-LC analysis (SCX, HILIC, high resolution size exclusion LC and high-pH reverse phase fractionation followed by direct online nano LC-MS and LC-MS/MS) for shotgun proteomics.  The system provides analytical-scale HPLC analysis at high flow rates, coupled with the high sensitivity hybrid quadrupole linear ion trap (4000 Q Trap) mass spectrometer, which can facilitate a broad range of studies, including high throughput small molecule quantitation studies. 

 


nanoACQUITY UPLC / SYNAPT HDMS (Q-IMS-TOF)

The (Q-IMS-TOF) SYNAPT HDMS (Waters) provides: (1) on-line LC-MS/MS for data-dependent protein ID and comparative proteomics; and (2) data-independent parallel MS/MS analysis of all precursor ions through an alternate scanning acquisition (LCMSE) mode for label-free quantitation of shotgun samples.  The integrated system has ion mobility separation capability based on gas-phase ion charges and shapes, for enhanced detection and identification of isobaric analytes.  With the capability of on-line desalting LC-MS or infusion MS under either denaturing or native conditions for intact protein molecular weight measurement, the integrated system enables protein-protein or protein-ligand noncovalent interaction analysis.  High mass accuracy allows for high confidence in the results of PTM analysis, de novo sequencing and small molecule profiling by LC-MS or LC-MS/MS.

The nanoACQULTY UPLC (Waters) provides direct nano-flow, offering significant improvements over conventional nano-flow separation technologies, with improved peak capacity and peak shape, and a larger number of components detected per separation.  The system’s 10,000 psi operating pressure capability allows for superior, high peak capacity separations by operating longer columns packed with sub-2 micron particles.  It is capable of 2D-LC separations at precise nano-flow rates.