EGFR signaling complexes responding to micro-patterned EGF surfaces
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Integrin b1 exhibits preferential localization at peripheral EGF-bound EGFR signaling complexes. NIH-3T3 (EGFR) cells plated onto micro-patterned EGF surfaces (red channel, lowest panel) were fixed and immunolabeled with an anti-β1 integrin primary antibody and A488-tagged secondary antibody (green channel, top panel), then immunolabeled with an anti-phosphotyrosine 4G10 antibody and A633-tagged secondary antibody (blue channel, middle panel). Panels display representative cell, and lowest panel shows merged images. Scale bar 10 µm.