Life Technologies Seminar: Ion Torrent Sequencing and Digital PCR
Thursday, March 6th, 2014
Start Time: 
11:00 AM
End Time: 
1:00 PM
Location / Facility: 
226 Weill Hall

Ion Torrent Sequencing
Dale Yuzuki, Ph.D.
Ion Torrent Market Development Manager
Thermo Fisher Scientific / Life Technologies
11:00 am - 12:00 pm

This presentation will cover:
•    Highly multiplexed PCR based Ion Torrent library preparation & rapid variant detection in a single workflow – SNP, Indels and CNV’s
•    New applications on Ion Proton sequencer and Personal Genome Machine
•    Quantitative targeted gene expression analysis up to 1,200 user defined genes in one amplification reaction
•    Custom AmpliSeq Designer Tool
•    Ion Reporter Bioinformatics Software – single, tumor & matched normal paired samples, trios….rich annotation & data filtering/mining tool

NGS Library Quantitation and CNV Studies with Digital PCR
Peter Schweitzer, Ph.D.
Director, BRC Genomics Facility
Biotechnology Resource Center (BRC)
Institute of Biotechnology
Cornell University, Ithaca, NY
12:00-12:00 pm

We are developing protocols to apply digital PCR for optimization of next generation sequencing (NGS) libraries.  The Life Technologies/ABI QuantStudio 3D digital PCR instrument allows quantification of NGS libraries by limiting dilution assays.  Up to 20,000 sub-nanoliter TaqMan PCR reactions can be performed on each chip.  We have designed these assays for NGS libraries.  Retrospectively, digital PCR results correlate well with NGS sequencing results.  Robust protocols are now being developed, with use of an internal reference for more accurate quantifications.  We are starting to use this digital PCR analysis prospectively for library quantifications in order to quantify individual libraries, prior to pooling, to balance the yield from each individual library in a pool, and to achieve more consistent cluster densities.  Case studies will be presented on using digital PCR for NGS library optimization and also for CNV studies.

Lunch will be served.

This seminar is hosted by the Cornell University Biotechnology Resource Center (BRC). 

To RSVP and to request more information, please contact Eugene Davoli or Luanne Lavender at Thermo Fisher Scientific / Life Technologies.

Biotechnology Development Facility
Biotechnology Resource Center (BRC)
Genomics Facility