Price List

Capillary DNA Analysis, ABI 3730xl instruments

Service Internal (Cornell and Cornell Affiliates) External External, NYSCAT subsidized (for-profit, New York State Companies ONLY)

Full Service Sequencing, plasmid or PCR product, per sample, template premixed with sequencing primer

$4.25 $6.80 $5.44

Special Conditions--dGTP addition, BAC sequencing, added to the single sample sequencing cost, per reaction

$5 $8 $6.40

Full Service Sequencing, plasmid or PCR product, per 96-well plate, templates premixed with sequencing primer, 94 samples

 $300  $480 $384
Ready-to-Load Sequencing, per 96-well plate  $42.50  $68 $54.40
Fragment analysis, ready-to-load, per 96-well plate $42.50 $68 $54.40

 

 

 

Illumina NextSeq500 sequencing

Service Internal (Cornell and Cornell Affiliates) External External, NYSCAT subsidized (for-profit, New York State Companies ONLY)
75 bp sequencing kit (1x75 bp or 2x37 bp) run $1,680 $2,688 $2,150
150 bp sequencing kit (1x150 bp or 2x75 bp) run $3,060 $4,896 $3,917
300 bp sequencing kit (2x150 bp) run $4,791 $7,665 $6,132

 

Illumina HiSeq 2500 sequencing (high-output mode) 8 lanes run with samples, no phiX lane

***Please note: unless you are going to submit 8 lanes of samples for sequencing, you should consider a Rapid Run flowcell, or a NextSeq run******

Service

Internal (Cornell and Cornell Affiliates)

External

External, NYSCAT subsidized (for-profit, New York State Companies ONLY)
Single-end, 50 bp sequencing lane  $1,103 $1,765 $1,412
Single-end, 100 bp sequencing lane  $1,403 $2,244 $1,795
Paired-end, 2x125 bp sequencing lane  contact us contact us contact us

 

Illumina HiSeq 2500 sequencing (rapid run mode)

Service

Internal (Cornell and Cornell Affiliates)

External

External, NYSCAT subsidized (for-profit, New York State Companies ONLY)

Single-end, 50 bp sequencing lane (one sample in both lanes) $1,749 $2,798 $2,239
Single-end, 100 bp sequencing lane (one sample in both lanes) $2,306 $3,689 $2,951
Single-end, 150 bp sequencing lane (one sample in both lanes) contact us contact us contact us
Paired-end, 2x100 bp sequencing lane (one sample in both lanes) $3,722 $5,956 $4,765
Paired-end, 2x150 bp sequencing lane (one sample in both lanes) $4,838 $7,740 $6,192
Paired-end, 2x200 bp sequencing lane (one sample in both lanes) contact us contact us contact us
Paired-end, 2x250 bp sequencing lane (one sample in both lanes) $6,232 $9,970 $7,976
Single-end, 50 bp sequencing lane (one sample, one lane), per lane $1,224 $1,959 $1,567
Single-end, 100 bp sequencing lane (one sample, one lane), per lane $1,503 $2,404 $1,923
Single-end, 150 bp sequencing lane (one sample, one lane), per lane contact us contact us contact us
Paired-end, 2x100 bp sequencing lane (one sample, one lane). per lane $2,211 $3,538 $2,830
Paired-end, 2x150 bp sequencing lane (one sample, one lane), per lane $2,769 $4,430 $3,544
Paired-end, 2x200 bp sequencing lane (one sample, one lane), per lane contact us contact us contact us
Paired-end, 2x250 bp sequencing lane (one sample, one lane), per lane $3,465 $5,544 $4,435

 

Illumina MiSeq sequencing

Service

Internal (Cornell and Cornell Affiliates)

External

External, NYSCAT subsidized (for-profit, New York State Companies ONLY)

50 bp sequencing kit (v2)* $902 $1,443 $1,155
300 bp sequencing kit (v2)* (e.g. 2 x 150 bp) $1,148 $1,837 $1,470
500 bp sequencing kit (v2)* (e.g. 2 x 250 bp) $1,293 $2,068 $1,654
150 bp sequencing kit (v3)* (e.g. 2 x 75 bp) $991 $1,586 $1,269
600 bp sequencing kit (v3)* (e.g. 2 x 300 bp) $1,653 $2,645 $2,116

* nominal read lengths--different length runs can be performed, depending on the type of sample/indexing used. For example, a 50 bp kit can be used for a single-end 50 bp sequencing run, or a paired-end 2 x 25 bp sequencing run. Also, if no index reads are required, a 50 bp kit can be used to sequence ~75 bp.

 

Illumina DNA/RNA Library Preps *

Service Internal (Cornell and Cornell Affiliates) External External, NYSCAT subsidized (for-profit, New York State Companies ONLY)

TruSeq DNA sample library prep, per sample *

$250 $400 $320

TruSeq RNA/small RNA sample library prep, per sample *

 $350  $560

$448

* Deluxe, standard Illumina TruSeq library prep service, generally for small numbers of samples. All QC/quantifications/size selections included. All efforts will be made to ensure success.

For larger numbers of samples, using modified protocols for affordable library preps, see Genomic Diversity Facility Price List, below.

 

Illumina data retrieval from archive

Service Internal (Cornell and Cornell Affiliates) External External, NYSCAT subsidized (for-profit, New York State Companies ONLY)

run data retrieval from archive

 $100 per flowcell/run $160 per flowcell/run $128 per flowcell/run

 

Real-time PCR/Sequence Detection System, ABI Viia7 instrument

Service Internal (Cornell and Cornell Affiliates) External       External, NYSCAT subsidized (for-profit, New York State Companies ONLY)

Real-time PCR run, per plate (96- or 384-well plate)

$10 per hour $16 per hour $12.80 per hour

 

Digital PCR, Bio-Rad QX100 instrument

Service Internal (Cornell and Cornell Affiliates) External External, NYSCAT subsidized (for-profit, New York State Companies ONLY)

digital PCR, Illumina library quantification (or others, not at the correct concentration) reactions set up by the Genomics facility

 $20 per sample run contact the Director of the Genomics Facility for pricing contact the Director of the Genomics Facility for pricing

digital PCR, DNA provided at correct concentration, not including cost of primers/probes, e.g. CNV, viral detection, reactions set up by the Genomics facility

$10 per sample run contact the Director of the Genomics Facility for pricing

contact the Director of the Genomics Facility for pricing

 

Instrument operation by user (training required). User provides all reagents and supplies.

 $20 per column read on QX100 (8 samples) contact the Director of the Genomics Facility for pricing contact the Director of the Genomics Facility for pricing

 

Illumina BeadArray Reader/SNP genotyping

Service Internal (Cornell and Cornell Affiliates) External External, NYSCAT subsidized (for-profit, New York State Companies ONLY)

Processing fee, 96 samples processed, does not include BeadChips/reagents from Illumina

contact the Director of the Genomics Facility contact the Director of the Genomics Facility contact the Director of the Genomics Facility

Processing fee, 48 samples processed, does not include BeadChips/reagents from Illumina

contact the Director of the Genomics Facility contact the Director of the Genomics Facility

contact the Director of the Genomics Facility

 

Agilent Microarrays

Service Internal (Cornell and Cornell Affiliates) External External, NYSCAT subsidized (for-profit, New York State Companies ONLY)

Sample QC, Labeling, Hybridization, and Scan

contact the Director of the Genomics Facility contact the Director of the Genomics Facility contact the Director of the Genomics Facility

Hybridization and Scan only

 contact the Director of the Genomics Facility contact the Director of the Genomics Facility

contact the Director of the Genomics Facility

Scan only

contact the Director of the Genomics Facility contact the Director of the Genomics Facility

contact the Director of the Genomics Facility

 

Sample preparation, QC, size selection, pooling:

Service Internal (Cornell and Cornell Affiliates) External External, NYSCAT subsidized (for-profit, New York State Companies ONLY)

Covaris sonication, per sample

free to use, contact us for details and training contact us contact us

Blue Pippin size selection, per sample

$20 $32 $25.60
Fragment Analyzer (RNA or DNA analysis), per sample $10 $16 $12.80

Pooling user-submitted Illumina libraries, per sample

(includes fragment analysis, Qubit quantification, and pooling)

$20 $32 $25.60

Pooling user-submitted Illumina libraries, per sample

(includes fragment analysis, Qubit quantification, digital PCR quantification, and pooling)

$40 $64 $51.20

 

 

Genomic Diversity Facility Price List

The Genomic Diversity Facility (GDF) is now a subdivision of the Genomics Facility and provides a high-throughput, low cost library preparation service for Illumina sequencing. Library types include 3'RNAseq libraries (minimum 48 samples), low cost Nextera skim sequencing libraries (minimum 95 samples), and TruSeq-equivalent libraries (minimum 8 samples).

To initiate a GDF project, or for more information, please email BRC_genomic-diversity@cornell.edu

 

Library preparation for Illumina Sequencing

Service Level Internal Internal
per
sample
External External
per
sample
NYS
company
NYS
per
sample
Mechanically sheared DNA WGS library preps* 8 samples $264 $33 $422.40 $52.80 $337.92 $42.24
  Plate** $2880 $30 $4608 $48 $3686.40 $38.40
Nextera skim sequencing WGS library preps
(1/3 concentration)
Plate $693.12 $7.22 $1,109 $11.55 $887.19 $9.24
Standard Nextera WGS library preps 8 samples $680 $85 $1,088 $136 $870.40 $108.80
  Plate $7,400 $77.08 $11,840 $123.33 $9,472 $98.67
NexteraXT-small genome WGS library preps 8 samples $350 $43.75 $560 $70 $448 $56
  Plate $3,800 $39.58 $6,080 $63.33 $4,864 $50.67
Amplicon barcoding (AmpSeq)
(Nextera or TruSeq indices)
Plate $1,500 $15.63 $2,400 $25 $1,920 $20
rAmpSeq library preps
(maize,sorghum,cassava)
Plate $240 $2.50 $384 $4 $307.20 $3.20
3'RNAseq library preps 48 samples $1,218 $25.38 $1,948.80 $40.60 $1,559 $32.48
  Plate $2,208 $23 $3,532.80 $36.80 $2,826.24 $29.44

* "Mechanically sheared DNA WGS library preps" are equivalent to TruSeq libraries, but with substituted reagents to reduce costs.
** 96 well plate including one blank negative control
 (empty well). For plates with fewer than 95 samples, a blank negative control is not required.

 

Other Genomic Diversity Facility Services*

Service Level Internal Internal
per
sample
External External
per
sample
NYS
company
NYS
per
sample
DNA extraction (plant leaf tissue only) Plate** $550 $5.79 inquire inquire inquire inquire
RNA extraction (limited tissues/species)*** Plate $500 $5.20 inquire inquire inquire inquire
DNA/RNA quality assurance              
DNA cleanup Plate $300 $3.16 inquire inquire inquire inquire
DNA quantification  Plate $100 $1.04 inquire inquire inquire inquire
RNA quantification Plate $125 $1.30 inquire inquire inquire inquire
 Tape Station RNA quality assessment Per sample $10 $10 inquire inquire inquire inquire
DNA/RNA sample dilution Plate $50 $0.53 inquire inquire inquire inquire
Sample re-array Plate $200 $2.11 inquire inquire inquire inquire
3'RNAseq quant. expression analysis 1 species
1 lane
$500 NA $800 NA $640 NA
Custom bioinformatics Hourly $70.60 NA $113 NA $90.40 NA
SNP calling (Tassel3-GBS pipeline) 1-2 plates $500 NA $800 NA $640 NA
  Additional
plates
$55 NA $88 NA $70.40 NA
Data retrieval from archive Per flowcell
lane
$100 NA $160 NA $128 NA
Hard drive shipping Drive $250 NA $400 NA $320 NA

* Prices may vary depending on project specifics. Most of the services in this table are for Cornell researchers only.
** 96 well plate including one blank negative control. A blank negative control (empty well) is required for a full plate of 96 samples.
*** Pilot study recommended for species/tissues we have not worked with previously.