Sample preparation

Sample preparation services include include 1D/2D gel and 1D/2D HPLC (SCX, SAX, HILIC, SEC and RP) separation, affinity enrichment/depletion, in-gel and in-solution digestion, sample desalting, sample concentration, and sample quality assessment/quality control (QA/QC), including:

  • 1D/2D gel separation with colloidal Coomassie blue or Sypro Ruby stain or ProQ Diamond or DIGE labeling.
  • Blue native gel and regular native gel
  • Gel imaging by Typhoon laser scanner
  • 1D/2D HPLC for separation of protein complexes under native or denaturing conditions and fraction collection
  • 1D/2D HPLC for complex sample fractionation
  • In-gel or in-solution enzymatic digestion and in-gel peptide extraction
  • Lectin-based affinity enrichment of glycoproteins and glycopeptides
  • Immuno and other affinity protein enrichment/depletion approaches
  • Phosphopeptide enrichment by IMAC or TiO2 beads
  • Sample desalting, sample concentration, protein quantitation