Our custom built multiphoton microscope (a technology invented at Cornell) enables confocal-like imaging using nonlinear optics, a technique which excels when imaging cloudy specimens (e.g., live tissues or mice), UV-excited fluorophores, or fibrillar collagen. This system is built on an inverted microscope with excitation from a 720-1000 nm Ti:Sapphire laser and emission spectrally separated into three channels by standard filter packs. Multiphoton microscopy is typically used in thick specimens (>100 um) using fluorescence from standard dyes or indicators, intrinsic fluorescence or second harmonic generation from macromolecular structures such as collagen fibrils or small non-centrosymmetric crystals.
Large samples can be accommodated on an upright stage. After an individual training and consultation session (contact), researchers are given access to schedule instrument time. Assistance with imaging and experimental design is available during normal business hours