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RBL (Rat Basophilic Leukemia) cells sensitized with A488-IgE (green panel) are plated on surfaces with patterned Cy3-DNP-BSA protein (red panel) and are subsequently fixed and labeled for F-actin using A647-phallodin (blue panel). Co-clustering of the IgE receptor (FcεRI) and actin cytoskeleton are observed at the sites of patterned antigen. Scale bar 10 µm.