NextSeq 2000 (Illumina) sequencing
The Illumina NextSeq 2000 (or 2k as we call it in house) is the most cost-effective sequencing platform currently in the Genomics Core.
- The NextSeq 2k uses patterned flowcells and Illumina’s ExAmp clustering chemistry, which require more stringent quality control prior to sequencing libraries.
- We also recommend using UDI (unique dual indexing) when prepping samples to be sequenced on this instrument. UDI is a known mitigation for filtering index-hopped reads seen in downstream analyses.
Illumina’s P2 flowcells produce a similar number of reads when compared with NextSeq 500/550 flowcells, but the smallest kit available for the P2 is 100 bp (vs. 75 bp for the NextSeq 500/550), which may be attractive for certain applications.
Illumina’s P3 flowcells have sequencing lanes that are physically larger than the P2 flowcells. This allows P3 flowcells to output around 1 billion fragments sequenced, or ~2.5 times the output of a NextSeq 2k or a NextSeq 500/550 flowcell.
|P2 kits||Reads passing filter* (M/flowcell)||Pass filter output (Gbp/flowcell)|
|P3 kits||Reads passing filter* (M/flowcell)||Pass filter output (Gbp/flowcell)|
This table shows nominal read lengths. Different length runs can be performed, depending on the type of sample/indexing used. Contact us prior to placing an order to discuss options.
* Values given for single-end reads. Paired-end mode would generate twice as many reads.
We provide a variety of read length options, as reflected in our price list. With the NextSeq 2k, runs can be configured with some flexibility. For example, a 50 bp kit can be used for a paired-end 2 x 25 bp run. In some cases, we may be able to offer custom read lengths. Please contact us to discuss options for your project.
Don't overpay for bad samples
If the QC results for the samples indicate poor quality and sequencing is not recommended, we will not process your samples. You will be charged only for the QC, not the sequencing of samples that are likely to fail.
|Description||Internal price (Cornell and Cornell affiliates)||External price|
|P2 100bp Cycles||$1,787||$2,931|
|P2 200bp Cycles||$3,360||$5,510|
|P2 300bp Cycles||$4,455||$7,306|
|P3 50bp Cycles||$2,723||$4,466|
|P3 100bp Cycles (e.g. 2 x 50)||$3,933||$6,450|
|P3 200bp Cycles (e.g. 2 x 100)||$5,445||$8,930|
|P3 300bp Cycles (e.g. 2 x 150)||$7,260||$11,906|