Capabilities
As a high-throughput sequencing system, the Illumina NextSeq500 instrument is recommended for whole-genome sequencing, exome sequencing, and RNA-Seq experiments.
- Currently, we offer read lengths 75 bp and 150 bp in single-end mode. In paired-end reads mode, we offer read length of 2 x 75 bp or 2 x 150 bp. These instruments can process one sample (or pool of samples) on a run.
- The NextSeq have 4 lanes per flow cell. However, libraries are loaded onto the flow cell from a single reservoir. You can sequence a single library or multiple pooled libraries on the flow cell.
- Depending on the application, the run time, and output per flowcell vary, as indicated in the tables below.
| High output flowcell |
Run time |
Reads passing filter (M/flowcell) |
Pass filter output (Gbp/flowcell) |
| Single-end 75 bp |
12 hours |
333-400 |
25-30 |
| Single-end 150 bp |
18 hours |
333-400 |
50-60 |
| Paired-end 2 x 75 bp |
18 hours |
666-800 |
50-60 |
| Paired-end 2 x 150 bp |
30 hours |
666-800 |
100-120 |
| Mid output flowcell |
Run time |
Reads passing filter (M/flowcell) |
Pass filter output (Gbp/flowcell) |
| Single-end 150 bp |
inquire |
130-200 |
19-30 |
| Paired-end 2 x 75 bp |
inquire |
260-400 |
19-30 |
| Paired-end 2 x 150 bp |
inquire |
260-400 |
38-60 |
Don't overpay for bad samples
When you place an order for sequencing of a library that you prepared with our Illumina Library small project pipeline, we automatically include an internal QC before sequencing your samples. If the QC results are very poor and sequencing is not recommended, we will not process your samples. You will be charged only for the QC, not the sequencing of samples that are likely to fail.
