NextSeq 500/550 (Illumina) sequencing
Capabilities
The Illumina NextSeq500 instruments are recommended for whole-genome sequencing, exome sequencing, and RNA-Seq experiments. These instruments can process one sample (or pool of samples) on a run.
Depending on the application, the run time, and output per flowcell vary, as indicated in the tables below.
| High-output flowcell | Reads passing filter* (M/flowcell) | Pass filter output (Gbp/flowcell) |
|---|---|---|
| 75 bp | 333-400 | 25-30 |
| 150 bp | 333-400 | 50-60 |
| 300 bp | 333-400 | 100-120 |
| Mid-output flowcell | Reads passing filter* (M/flowcell) | Pass filter output (Gbp/flowcell) |
|---|---|---|
| 150 bp | 130-200 | 19-30 |
| 300 bp | 130-200 | 38-60 |
This table shows nominal read lengths. Different length runs can be performed, depending on the type of sample/indexing used. Contact us prior to placing an order to discuss options.
* Values given for single-end reads. Paired-end mode would generate twice as many reads.
Flexibility
We provide a variety of read length options, as reflected in our price list. With the Illumina 500/550 instruments, runs can be configured with some flexibility. For example, a 50 bp kit can be used for a paired-end 2 x 25 bp run. In some cases, we may be able to offer custom read lengths. Please contact us to discuss options for your project.
Don't overpay for bad samples
If the QC results for the samples indicate poor quality and sequencing is not recommended, we will not process your samples. You will be charged only for the QC, not the sequencing of samples that are likely to fail.
Service pricing
| Description | Internal price (Cornell and Cornell affiliates) | External price |
|---|---|---|
| High-output kit, 75 bp | $1,848 | $3,031 |
| High-output kit, 150 bp | $3,366 | $5,520 |
| High-output kit, 300bp | $5,270 | $8,643 |
| Mid-output kit, 150 bp | $1,166 | $1,912 |
| Mid-output kit, 300 bp | $1,869 | $3,065 |