Skip to main content

NextSeq 500/550 (Illumina) sequencing

Capabilities

As a high-throughput sequencing system, the Illumina NextSeq500 instrument is recommended for whole-genome sequencing, exome sequencing, and RNA-Seq experiments.

  • Currently, we offer read lengths 75 bp and 150 bp in single-end mode. In paired-end reads mode, we offer read length of 2 x 75 bp or 2 x 150 bp. These instruments can process one sample (or pool of samples) on a run.
  • The NextSeq have 4 lanes per flow cell. However, libraries are loaded onto the flow cell from a single reservoir. You can sequence a single library or multiple pooled libraries on the flow cell.
  • Depending on the application, the run time, and output per flowcell vary, as indicated in the tables below.
High output flowcell Run time Reads passing filter (M/flowcell) Pass filter output (Gbp/flowcell)
Single-end 75 bp 12 hours 333-400 25-30
Single-end 150 bp 18 hours 333-400 50-60
Paired-end 2 x 75 bp 18 hours 666-800 50-60
Paired-end 2 x 150 bp 30 hours 666-800 100-120
Mid output flowcell Run time Reads passing filter (M/flowcell) Pass filter output (Gbp/flowcell)
Single-end 150 bp inquire 130-200 19-30
Paired-end 2 x 75 bp inquire 260-400 19-30
Paired-end 2 x 150 bp inquire 260-400 38-60

Don't overpay for bad samples

When you place an order for sequencing of a library that you prepared with our Illumina Library small project pipeline, we automatically include an internal QC before sequencing your samples. If the QC results are very poor and sequencing is not recommended, we will not process your samples. You will be charged only for the QC, not the sequencing of samples that are likely to fail.

Service pricing

NextSeq 500/550 (Illumina) sequencing
DescriptionInternal price (Cornell and Cornell affiliates)External price
75 bp sequencing kit (1x75 bp or 2x37 bp) run $1,848 $3,031
150 bp sequencing kit (1x150 bp or 2x75 bp) run $3,366 $5,520
300 bp sequencing kit (2x150 bp) run $5,270 $8,643
150 bp Mid output kit, ~130 M clusters sequenced* $1,166 $1,912
300 bp Mid output kit, ~130 M clusters sequenced* $1,869 $3,065
* smaller output kits, usually not kept in stock