This service will determine the size profile of RNA or DNA samples, including primary biological samples and NGS libraries or pools.

The size profile of different sample types indicates: 

• RNA integrity (degree of degradation), reflected in the RQN value. RQN>7 is considered intact.
• The average size for NGS libraries, which can be used to calculate the molarity.
• The average size of genomic DNA samples.

Based on the concentration and expected size distribution of your samples, select the appropriate Agilent instrument for your project when placing an order.

Fragment Analyzer – used for most samples

Femto Pulse – used to determine the size of high molecular weight genomic DNA and for very low concentration RNA samples.

Please dilute samples to the appropriate concentration range before submission. Samples that are too concentrated or too dilute will impact the results of the QC assay. For more information about how to dilute samples, please click the link below for “Sample Preparation and Shipping”.

The results will include the size distribution profile as well as quantitative metrics for called peaks. The reported sample concentration may not be accurate and a Qubit or Nanodrop/Quickdrop reading is the preferred method to determine sample concentration. Samples containing salts or other impurities that affect electrophoretic mobility may not have accurate sizing with this assay.
 

Sample preparation and shipping

Fragment Analyzer result interpretation