RNA-Seq Standard package
- Sample submission: 10ng — 1ug purified RNA (see below).
‘Bioanalyzer QC’ is highly recommended; contact us for information about RNA QC options. - Library preparation: NEBNext Ultra II [Directional] RNA
PolyA* enrichment (included) or ribosomal RNA depletion (extra cost; we can deplete animal and/or bacterial rRNA). - Directional (stranded, default) or non-directional (non-stranded, recommended for input < 20ng total RNA).
- Sequencing: minimum 10M raw reads. 10M and 20M read packages available.
10M read package is recommended for Bacterial sequencing.
Deeper sequencing is available on request (extra cost). - Standard analysis: Read preprocessing, mapping to reference genome, data QC reports including mapping rate and sample
clustering analyses, differential gene expression analysis (DESeq2).
Alternative analysis modes including pathogen discovery are also available; contact us for information.
Service pricing
| Description | Internal price (Cornell and Cornell affiliates) | External Price |
|---|---|---|
| RNAseq package (20M reads) Samples 1-24 (ea.) | $280 | Academic/Non-Profit: $350 / Industry: $460 |
| RNAseq package (20M reads) Samples 25+ | $230 | Academic/Non-Profit: $290 / Industry: $380 |
| RNAseq package (10M reads) Samples 1-24 (ea.) | $245 | Academic/Non-Profit: $310 / Industry: $405 |
| RNAseq package (10M reads) Samples 25+ | $195 | Academic/Non-Profit: $250 / Industry: $325 |
| low-input RNAseq package (20M reads), samples 1-24 | $350 | Academic/Non-Profit: $440 / Industry: $575 |
| low-input RNAseq package (20M reads), samples 25+ | $305 | Academic/Non-Profit: $385 / Industry: $500 |
| add rRNA Subtraction (per sample) | $65 | Academic/Non-Profit: $80 / Industry: $105 |
| add 10M additional reads | $90 | Academic/Non-Profit: $115 / Industry: $150 |
| add 20M additional reads | $125 | Academic/Non-Profit: $155 / Industry: $205 |
| Description | Internal price (Cornell and Cornell affiliates) | External Price |
|---|---|---|
| RNA sample cleanup (per sample) | $45 | Academic/Non-Profit: $55 / Industry: $75 |
| Fragment Analyzer (RNA analysis) - per sample | $18 | Academic/Non-Profit: $23 / Industry: $30 |
| FEMTO Pulse (RNA analysis) - per sample | $24 | Academic/Non-Profit: $30 / Industry: $39 |
| TSO cDNA amplification (per sample) | $100 | Academic/Non-Profit: $125 / Industry: $165 |
| Custom lab work (per hour) | $65 | Academic/Non-Profit: $105 / Industry: $105 |
| Consultation and custom data analysis (per hour) | $20 | Academic/Non-Profit: $25 / Industry: $35 |
| Custom projects are available on a limited basis and require pre-approval | ||