Small RNA Sequencing Standard Package
- Sample submission: 100ng - 1ug purified RNA (lower input is sufficient for cell-free RNA; see below).
‘Bioanalyzer QC’ is highly recommended; contact us for information about RNA QC options. - Library preparation: NEBNext Small RNA.
- Sequencing: minimum 10M raw reads.
Deeper sequencing is available on request (extra cost). - Standard analysis: Read preprocessing, quantification of annotated microRNAs (miRdeep2 using miRBase annotations), data QC
reports including total microRNA counts and sample clustering analyses, differential microRNA detection analysis (DESeq2).
Optional: reads can be mapped to a reference genome on request.
Service pricing
| Description | Internal price (Cornell and Cornell affiliates) | External Price |
|---|---|---|
| Samples 1-24 (ea.) | $280 | Academic/Non-Profit: $355 / Industry: $460 |
| Samples 25+ (ea.) | $230 | Academic/Non-Profit: $290 / Industry: $380 |
| add 10M additional reads | $90 | Academic/Non-Profit: $115 / Industry: $150 |
| Description | Internal price (Cornell and Cornell affiliates) | External Price |
|---|---|---|
| RNA sample cleanup (per sample) | $45 | Academic/Non-Profit: $55 / Industry: $75 |
| Fragment Analyzer (RNA analysis) - per sample | $18 | Academic/Non-Profit: $23 / Industry: $30 |
| Custom lab work (per hour) | $65 | Academic/Non-Profit: $105 / Industry: $105 |
| Consultation and custom data analysis (per hour) | $125 | Academic/Non-Profit: $205 / Industry: $205 |
| Custom projects are available on a limited basis and require pre-approval | ||