Fluorescence Activated Cell Sorting (FACS) enables researchers to sort suspended cells according to their individual fluorescence characteristics, reported by cell surface antibody markers, genetically-introduced fluorescent proteins or exogenously-introduced vital indicators. Cells can be fixed or live, and sorting can be accomplished by gating regions from a broad spectrum of channels. Our new BD FACSAria Fusion flow cytometry instrument currently supports twelve emission colors off of four excitation laser lines. Cells can be sorted into tubes or multi-well plates, as dictated by the downstream analysis. This instrument is isolated in a biosafety cabinet and can safely handle risk-level-2 agents.