Skip to main content

Cornell University

Epigenomics Facility

Contact

What We Offer

Service Workflow

What we offer

The BRC Epigenomics Facility’s (EGC) specialty is the ChIP-exo assay, an ultra-high-resolution version of ChIP-seq that identifies protein binding to DNA at specific sites genome-wide. Our expertise in mapping protein-DNA binding sites is especially useful for researchers interested in identifying novel DNA motifs bound by their protein of interest, assessing changes in transcription factor binding under different environmental or genetic conditions, and mapping nucleosome positions or modifications.

We process mammalian tissue samples, cell lines, yeast, and bacteria, and our full-service ChIP-exo assay includes all library preparation, sequencing, and basic bioinformatics (demultiplexing and genome alignment) with approximately a 2-month turnaround time. Researchers can provide their own antibodies against desired protein targets or pay the fractional cost per ChIP for antibodies against a limited set of popular targets (Histone H3, H3K27ac, H3K4me3, RNA Polymerase II, CTCF, TBP). TAP tags, HA tags, and Myc tags are also compatible with the ChIP-exo workflow. Interested researchers should schedule a free consultation with the Epigenomics team by contacting brc_epigenomics@cornell.edu!

The EGC provides a high-throughput end-to-end service that maps genome-wide protein-DNA interactions, tracks experimental metadata, and implements quality-controlled data processing and research-based analysis pipelines. We provide epigenomic and bioinformatic research resources and services that include sample preparation services and data generation. Our open-source platforms enable and reinforce FAIR data practices. The core is able to receive and process cell and tissue samples for various diagnostic epigenetic assays.

Acknowledging BRC services and staff

If you publish data that has been generated with the help of the services we provide, mentioning the Cornell Institute of Biotechnology in the acknowledgments section is very helpful. Here is why. You can also review our authorship guidelines. please reference the following research Resource Identifiers (RRIDs):

group photo