Price List

Capillary DNA Analysis, Sanger Sequencing, Fragment Analysis, ABI 3730xl instruments

Illumina NextSeq500 sequencing

Illumina HiSeq 2500 sequencing (high-output mode) 8 lanes run with samples, no phiX lane

Illumina HiSeq 2500 sequencing (rapid run mode)

Illumina MiSeq sequencing

Illumina DNA/RNA Library Preps 

Real-time PCR/Sequence Detection System, ABI Viia7 instrument

Digital PCR, Bio-Rad QX100 instrument

Illumina BeadArray Reader/SNP genotyping

Sample preparation, QC, size selection, pooling (Covaris, Blue Pippin, Fragment Analyzer etc...)

High-throughput Library preparation for Illumina Sequencing and Full Service Projects

Other Genomics Facility Services


Capillary DNA Analysis, Sanger Sequencing, Fragment Analysis, ABI 3730xl instruments

Service Internal (Cornell and Cornell Affiliates) External External, NYSCAT subsidized (for-profit, New York State Companies ONLY)

Full Service Sequencing, plasmid or PCR product, per sample, template premixed with sequencing primer

$4.25 $6.97 $5.58

Special Conditions--dGTP addition, BAC sequencing, added to the single sample sequencing cost, per reaction

$5 $8.20 $6.56

Full Service Sequencing, plasmid or PCR product, per 96-well plate, templates premixed with sequencing primer, 94 samples

 $300  $492 $393.60
Ready-to-Load Sequencing, per 96-well plate  $42.50  $69.70 $55.80
Fragment analysis, ready-to-load, per 96-well plate $42.50 $69.70 $55.80
LIZ500 and HiDi Formamide addition, per 96-well plate $40.00 $65.60 $52.48

 


Illumina NextSeq500 sequencing

Service Internal (Cornell and Cornell Affiliates) External External, NYSCAT subsidized (for-profit, New York State Companies ONLY)
75 bp sequencing kit (1x75 bp or 2x37 bp) run $1,680 $2,755 $2,204
150 bp sequencing kit (1x150 bp or 2x75 bp) run $3,060 $5,018 $4,015
300 bp sequencing kit (2x150 bp) run $4,791 $7,857 $6,286
Smaller output kits (usually not kept in stock):      
150 bp Mid output kit, ~130 M clusters sequenced $1,060 $1,738 $1,391
300 bp Mid output kit, ~130 M clusters sequenced $1,699 $2,786 $2,229

 


Illumina HiSeq 2500 sequencing (rapid run mode)

Service

Internal (Cornell and Cornell Affiliates)

External

External, NYSCAT subsidized (for-profit, New York State Companies ONLY)

Single-end, 50 bp sequencing lane (one sample in both lanes) $1,749 $2,868 $2,295
Single-end, 100 bp sequencing lane (one sample in both lanes) $2,306 $3,782 $3,025
Single-end, 150 bp sequencing lane (one sample in both lanes) contact us contact us contact us
Paired-end, 2x100 bp sequencing lane (one sample in both lanes) $3,722 $6,104 $4,883
Paired-end, 2x150 bp sequencing lane (one sample in both lanes) $4,838 $7,934 $6,347
Paired-end, 2x200 bp sequencing lane (one sample in both lanes) contact us contact us contact us
Paired-end, 2x250 bp sequencing lane (one sample in both lanes) $6,232 $10,220 $8,176
Single-end, 50 bp sequencing lane (one sample, one lane), per lane $1,224 $2,007 $1,606
Single-end, 100 bp sequencing lane (one sample, one lane), per lane $1,503 $2,465 $1,972
Single-end, 150 bp sequencing lane (one sample, one lane), per lane contact us contact us contact us
Paired-end, 2x100 bp sequencing lane (one sample, one lane). per lane $2,211 $3,626 $2,901
Paired-end, 2x150 bp sequencing lane (one sample, one lane), per lane $2,769 $4,541 $3,633
Paired-end, 2x200 bp sequencing lane (one sample, one lane), per lane contact us contact us contact us
Paired-end, 2x250 bp sequencing lane (one sample, one lane), per lane $3,565 $5,847 $4,677

 


Illumina HiSeq 2500 sequencing (high-output mode) 8 lanes run

***Please note: you must submit 8 lanes of samples for this type of sequencing run******

Service

Internal (Cornell and Cornell Affiliates)

External

External, NYSCAT subsidized (for-profit, New York State Companies ONLY)
Single-end, 50 bp sequencing lane  $8,824 (all 8 lanes) $14,471 (all 8 lanes) $11,577 (all 8 lanes)
Single-end, 100 bp sequencing lane  $11,224 (all 8 lanes) $18,407 (all 8 lanes) $14,725 (all 8 lanes)
Paired-end, 2x125 bp sequencing lane  contact us contact us contact us

 



Illumina MiSeq sequencing

Service

Internal (Cornell and Cornell Affiliates)

External

External, NYSCAT subsidized (for-profit, New York State Companies ONLY)

50 bp sequencing kit (v2)* $902 $1,479 $1,183
300 bp sequencing kit (v2)* (e.g. 2 x 150 bp) $1,148 $1,883 $1,506
500 bp sequencing kit (v2)* (e.g. 2 x 250 bp) $1,293 $2,121 $1,696
150 bp sequencing kit (v3)* (e.g. 2 x 75 bp) $991 $1,625 $1,300
600 bp sequencing kit (v3)* (e.g. 2 x 300 bp) $1,653 $2,711 $2,169
Smaller output kits (usually not kept in stock):      
300 bp Nano kit, ~1 M clusters sequenced $292 $479 $383
500 bp Nano kit, ~1 M clusters sequenced $350 $574 $460
300 bp Micro kit, ~4 M clusters sequenced $438 $718 $575

* nominal read lengths--different length runs can be performed, depending on the type of sample/indexing used. For example, a 50 bp kit can be used for a single-end 50 bp sequencing run, or a paired-end 2 x 25 bp sequencing run. Also, if no index reads are required, a 50 bp kit can be used to sequence ~75 bp.

 


Illumina DNA/RNA Library Preps *

Service Internal (Cornell and Cornell Affiliates) External External, NYSCAT subsidized (for-profit, New York State Companies ONLY)

TruSeq DNA nano library prep (Illumina Kit), per sample, samples 1-8 *

$200 $328 $262

                                                                                samples 9-24 *

$100 $164 $131

                                                                                samples 25-96 *

$90 $148 $118

PCR-free TruSeq DNA nano library prep (Illumina kit), per sample, samples 1-8 *

$175 $287 $230

                                                                                samples 9-24 *

$100 $164 $131

                                                                                samples 25-96 *

$85 $139 $112

NextFLEX library prep (Illumina kit), per sample, samples 1-8 *

$150 $246 $197

                                                                     samples 9-24 *

$100 $164 $131

                                                                     samples 25-96 *

$80 $131 $105

TruSeq RNA, stranded sample library prep (Illumina kit), per sample, samples 1-8 *

 $300  $492

$394

                                                                               samples 9-24 *

$160 $262

$210

                                                                               samples 25-96 *

$125 $205

$164

* Deluxe, standard Illumina TruSeq library prep service, generally for small numbers of samples. All QC/quantifications/size selections included. All efforts will be made to ensure success.

For larger numbers of samples, using modified protocols for affordable library preps, see High-throughput Library preparation for Illumina Sequencing and Full Service Projects, below.

 


Real-time PCR/Sequence Detection System, ABI Viia7 instrument

Service Internal (Cornell and Cornell Affiliates) External       External, NYSCAT subsidized (for-profit, New York State Companies ONLY)

Real-time PCR run, per plate (96- or 384-well plate)

$10 per hour $16 per hour $12.80 per hour

 


Digital PCR, Bio-Rad QX100 instrument

Service Internal (Cornell and Cornell Affiliates) External External, NYSCAT subsidized (for-profit, New York State Companies ONLY)

digital PCR, Illumina library quantification (or others, not at the correct concentration) reactions set up by the Genomics facility

 $20 per sample run contact the Director of the Genomics Facility for pricing contact the Director of the Genomics Facility for pricing

digital PCR, DNA provided at correct concentration, not including cost of primers/probes, e.g. CNV, viral detection, reactions set up by the Genomics facility

$10 per sample run contact the Director of the Genomics Facility for pricing

contact the Director of the Genomics Facility for pricing

 

Instrument operation by user (training required). User provides all reagents and supplies.

 $20 per column read on QX100 (8 samples) contact the Director of the Genomics Facility for pricing contact the Director of the Genomics Facility for pricing

 


 

 


Agilent Microarrays

Service Internal (Cornell and Cornell Affiliates) External External, NYSCAT subsidized (for-profit, New York State Companies ONLY)

Scan only

contact the Director of the Genomics Facility contact the Director of the Genomics Facility

contact the Director of the Genomics Facility

 


Sample preparation, QC, size selection, pooling

Service Internal (Cornell and Cornell Affiliates) External External, NYSCAT subsidized (for-profit, New York State Companies ONLY)

Covaris sonication, per sample

free to use, contact us for details and training contact us contact us

Blue Pippin size selection, per sample

$20 $32 $25.60
Fragment Analyzer (RNA or DNA analysis), per sample $10 $16 $12.80

Pooling user-submitted Illumina libraries, per sample

(includes fragment analysis, Qubit quantification, and pooling)

$20 $32 $25.60

Pooling user-submitted Illumina libraries, per sample

(includes fragment analysis, Qubit quantification, digital PCR quantification, and pooling)

$40 $64 $51.20

 


High-throughput Library preparation for Illumina Sequencing and Full Service Projects

To initiate a project, or for more information, please email BRC_genomics-projects@cornell.edu

Service Level Internal External NYS
company
TruSeq-equivalent WGS library preps
(custom protocol with PCR)
48 samples* $2,851 per half plate $4,676 $3,741
  96 samples* $3,802 per full plate $6,236 $4,989
Nextera skim sequencing WGS library preps
(1/3 concentration)
48 samples* $864 per half plate $1,417 $1,134
  96 samples* $1,152 per full plate $1,890 $1,512
Nextera XT small genome WGS library preps
(full volume Illumina protocol)
48 samples* $3,888 per half plate $6,377 $5,102
  96 samples* $5,184 per full plate $8,502 $6,802
Low-volume Nextera XT small genome WGS library preps
(1/5 volume reactions)
48 samples* $1,728 per half plate $2,834 $2,268
  96 samples* $2,304 per full plate $3,779 $3,023
3'RNAseq library preps 24 samples $1,490 per quarter plate $2,444 $1,955
  48 samples $1,987 per half plate $3,259 $2,607
  96 samples* $2,650 per full plate $4,346 $3,477
Amplicon barcoding (AmpSeq)
(Nextera or TruSeq indices)
96 samples* $250 per full plate $410 $328
rAmpSeq library preps
(maize,sorghum,cassava)
96 samples* $288 per full plate $473 $378

* These numbers include one blank well

 


Other Genomics Facility Services*

Service Level Internal External NYS
company
DNA extraction (plant leaf tissue only) Plate** $550 inquire inquire
RNA extraction (limited tissues/species)*** Plate** $500 inquire inquire
DNA/RNA quality assurance        
DNA cleanup Plate $300 inquire inquire
DNA quantification  Plate $100 inquire inquire
RNA quantification Plate $125 inquire inquire
 Tape Station DNA/RNA quality assessment Per sample $10 inquire inquire
DNA/RNA sample dilution Plate $50 inquire inquire
Sample re-array Plate $200 inquire inquire
Extra/custom pooling of Illumina libraries Per pool $60 $99 $80
3'RNAseq bioinformatics (gene counting) 1 species
1 lane
$500 $820 $656
Custom bioinformatics Hourly $80 $132 $106
SNP calling (Tassel3) 1-2 plates $500 $820 $656
  Additional
plates
$55 $91 $73
Illumina data retrieval from archive Per lane $100 $164 $132
Sanger data retrieval from archive Per read $5 $8.20 $6.56
Hard drive shipping Drive $250 $410 $328

* Prices may vary depending on project specifics. Many of the services in this table are for Cornell researchers only.
** 96 well plate including one blank negative control. A blank negative control (empty well) is required for a full plate of 96 samples.
*** Pilot study recommended for species/tissues we have not worked with previously.