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Cornell University

RNA-Seq Standard package

  • Sample submission: 10ng — 1ug purified RNA (see below).
    ‘Bioanalyzer QC’ is highly recommended; contact us for information about RNA QC options.
  • Library preparation: NEBNext Ultra II [Directional] RNA
    PolyA* enrichment (included) or ribosomal RNA depletion (extra cost; we can deplete animal and/or bacterial rRNA).
  • Directional (stranded, default) or non-directional (non-stranded, recommended for input < 20ng total RNA).
  • Sequencing: minimum 20M raw reads.
    Deeper sequencing is available on request (extra cost).
  • Standard analysis: Read preprocessing, mapping to reference genome, data QC reports including mapping rate and sample
    clustering analyses, differential gene expression analysis (DESeq2).
    Alternative analysis modes including pathogen discovery are also available; contact us for information.

Service pricing

RNA-Seq Standard package
DescriptionInternal price (Cornell and Cornell affiliates)External Price
Samples 1-24 (ea.) $315 $470
Samples 25+ (ea.) $265 $470
add rRNA Subtraction (per sample) $50 $100
add 20M Additional Reads $160 $255
Custom Services
Custom Lab WorkCustom Analysis
$45/hr $125/hr
Custom projects are available on a limited basis and require pre-approval from the Director of the TREx Facility