RNA-Seq Standard package
- Sample submission: 10ng — 1ug purified RNA (see below).
‘Bioanalyzer QC’ is highly recommended; contact us for information about RNA QC options. - Library preparation: NEBNext Ultra II [Directional] RNA
PolyA* enrichment (included) or ribosomal RNA depletion (extra cost; we can deplete animal and/or bacterial rRNA). - Directional (stranded, default) or non-directional (non-stranded, recommended for input < 20ng total RNA).
- Sequencing: minimum 20M raw reads.
Deeper sequencing is available on request (extra cost). - Standard analysis: Read preprocessing, mapping to reference genome, data QC reports including mapping rate and sample
clustering analyses, differential gene expression analysis (DESeq2).
Alternative analysis modes including pathogen discovery are also available; contact us for information.
Service pricing
| Description | Internal price (Cornell and Cornell affiliates) | External Price |
|---|---|---|
| Samples 1-24 (ea.) | $315 | $470 |
| Samples 25+ (ea.) | $265 | $470 |
| add rRNA Subtraction (per sample) | $50 | $100 |
| add 20M Additional Reads | $160 | $255 |
| Custom Lab Work | Custom Analysis |
|---|---|
| $55/hr | $125/hr |
| Custom projects are available on a limited basis and require pre-approval from the Director of the TREx Facility | |