Small RNA Sequencing Standard Package
- Sample submission: 100ng - 1ug purified RNA (lower input is sufficient for cell-free RNA; see below).
‘Bioanalyzer QC’ is highly recommended; contact us for information about RNA QC options. - Library preparation: NEBNext Small RNA.
- Sequencing: minimum 10M raw reads.
Deeper sequencing is available on request (extra cost). - Standard analysis: Read preprocessing, quantification of annotated microRNAs (miRdeep2 using miRBase annotations), data QC
reports including total microRNA counts and sample clustering analyses, differential microRNA detection analysis (DESeq2).
Optional: reads can be mapped to a reference genome on request.
Service pricing
Description | Internal price (Cornell and Cornell affiliates) | External Price |
---|---|---|
Samples 1-24 (ea.) | $265 | $375 |
Samples 25+ (ea.) | $215 | $375 |
Add 10M Additional Reads | $110 | $160 |
Custom Lab Work | Custom Analysis |
---|---|
$55/hr | $125/hr |
Custom projects are available on a limited basis and require pre-approval from the Director of the TREx Facility |