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Cornell University

Small vs. large project pipelines

Graphical representation of steps in small and large project library preparation pipelines

Side-by-side comparison of two pipelines. On the left side of the image, the pipeline for large sequencing projects, and on the right side of the image, the pipeline for small sequencing projects. Steps for the large projects pipeline, from top to bottom:

  1. DNA/RNA extraction,
  2. DNA/RNA cleanup
  3. Concentration assessment
  4. Optional sample normalization (concentration or dilution)
  5. Library preparation on a subset of samples
  6. In case of success, library preparation on all the samples; 6') in case of failure, check fragment size and concentration,
  7. Pooling
  8. Optional custom or extra pooling
  9. Sequencing at BRC
  10. Library shipped to customer

Steps 1, 2, 4, 8, and 9 are marked with a star that denotes services that can be ordered alone. Steps 3 to 7 are included when a user orders a library preparation.

Steps for the small projects pipeline, from top to bottom:

  1. User submits extracted and cleaned-up DNA/RNA
  2. DNA/RNA quality control (purity and integrity assessment). In case of failure, the sample us sent back to customer. In case of success, go to step 3.
  3. DNA/RNA quantification
  4. Library preparation
  5. Library QC
  6. Optional Size selection (+ new QC)
  7. Library quantification, with 7a. Qubit, 7b. digital PCR
  8. Pooling
  9. Digital PCR and sequencing.

Steps 2, 3, 5, 7, and 9 are marked with a star that denotes services that can be ordered alone. Steps 2 to 7 are included when a user orders a library preparation. Steps 5 to 8 are included when a user orders a library pooling.