Top-down proteomics measure the molecular weight of intact proteins while conduct direct protein sequencing without need for protein digestion.
By working on the true proteoforms, top-down proteomics identifies intact protein sequences, attributes the modifications to each protein. It gives you information about the combined proteoforms, with which modifications it carries, and on which sites they are.
With top-down proteomics, you go beyond knowing how many modifications you have at how many sites, and you achieve levels of details that cannot be achieved by traditional bottom-up (at peptide level) techniques.
If you are interested in top-down proteomics, contact us!