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Library pooling

Indexing

For multiple samples to be pooled into a single lane, they need to be indexed (barcoded). This can be done using either commercial or custom indexing.

  • If you used Illumina TruSeq or Nextera indices -> We will be able to separate the reads and provide .fastq files for each individual sample.
  • If you used custom indexing adaptors -> You will need to provide us with the sequences prior to the run. You will be responsible for sorting the reads that belong to each sample yourself.
If you did your library preparation with us, either for a small or large project, all QC and pooling are already included in our library preparation pipeline.

Understanding the price table

Quality control

  1. If you prepared your library and submit your samples for pooling, we will include standard QC before pooling, including sizing and quality assessment with our Fragment Analyzer, and quantification with our Qubit.
  2. Optionally, we can add a digital PCR quantification step for extra accuracy before pooling.

Extra or custom pooling

Pooling and QC are included in library preparation pipelines (both for small and large projects). However, if you need a custom or extra pooling, we offer this service alone.

Service pricing

Library pooling
DescriptionInternal price (Cornell and Cornell affiliates)External price
Pooling user-submitted Illumina libraries, per sample (includes fragment analysis, Qubit quantification, and pooling) $24 $39
Pooling user-submitted Illumina libraries, per sample (includes fragment analysis, Qubit quantification, digital PCR quantification, and pooling) $48 $79
Extra/custom pooling of Illumina libraries (per pool) $72 $118