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Quantitative (comparative) proteomics

We offer both labeled and label-free services:

Categories Methods Multiplex Features Main limitation

Labeled

SILAC

3

Most accurate, MS-based quantitation

Requiring cell culture for labeling

Dimethyl

3

MS-based quantitation suitable for limited materials; flexibility

Limit for 3-plex

iTRAQ

4 or 8

Excellent MS/MS-based quantitation; flexibility

Ratio compression

TMT

6

Excellent MS/MS-based quantitation; flexibility

Ratio compression

Label-free

XIC

1

MS-based precursor ion intensities or peak areas

Large quantitation variation, requiring optimized LCs

Spectral counting or emPAI

1

MS/MS-based spectra counts or pep ID numbers

Large quantitation variation, requiring optimized LCs

SRM

1

Super-accurate MS/MS quantitation

Requiring synthesis of standard peptides and known targeted peptides

Our full service includes sample preparation, sample analysis and data interpretation. If you want to do the sample preparation or data interpretation yourself, let us know during the project consultation.

In addition, we provide equipment and software support for 2D gel-based quantitation analysis (2DE or 2D DIGE), upon requested for some specific projects.

The service fees are broken down in the tables below.

Service pricing

Sample preparation
DescriptionInternal price (Cornell and Cornell affiliates)External price
Bradford Assay (set up) $40 $64
Bradford Assay (per sample) $5 $8
Affinity depletion of serum high abundant proteins (per sample) $120 $192
iTRAQ 4-plex reagent (per set of samples) $280 $448
iTRAQ 4-plex labeling and sample preparation (per set of samples) $500 $800
iTRAQ 8-plex reagent (per set of samples) $560 $896
iTRAQ 8-plex labeling and sample preparation (per set of samples) $700 $1,120
TMT 6-plex reagent (per set of samples) $380 $608
TMT 6-plex labeling and sample preparation (per set of samples) $600 $960
TMT10-plex reagent (per set of samples) $750 $1,200
TMT 10-plex labeling and sample preparation (per set of samples) $800 $1,280
TMT 11-plex reagent (per set of samples) $880 $1,408
TMT 11-plex labeling and sample preparation (per set of samples) $880 $1,408
TMT 16-plex reagent (per set of samples) $1,500 $2,400
TMT 16-plex labeling and sample preparation (per set of samples) $1,300 $2,080
Buffer exchange by ultrafiltration/dialysis/spin column (per sample) $25 $40
Spin filtration (per sample) $15 $24
Gel-based protein quantitation for shotgun (per set of samples) $200 $320
dimethylation duplex labeling (per set of samples) $350 $560
dimethylation triplex labeling (per set of samples) $450 $720
mini SDS-PAGE, precast (per gel) $85 $136
Gel imaging (per hour) $60 $96
ImageQuant analysis for 1D gel bands (per gel) $50 $80
In-solution digestion (set up) $100 $160
In-solution digestion (per sample) $50 $80
Strap supplier for insolution digestion (per sample) $10 $16
MCX/MAX Cartridge (manual) (per sample) $60 $96
HRP manual fractionation (per sample) $100 $160
HPLC/MS
DescriptionInternal price (Cornell and Cornell affiliates)External price
high pH reverse phase with fractions collection (per sample) $250.00 $400.00
nanoLC-MS/MS: 90-120min gradient in Orbitrap Fusion/Eclipse (per sample) $250 $400
nanoLC-MS/MS: 90-120min gradient in Orbitrap Eclipse RTS (per sample) $300 $480
MRM-LC-MS/MS for peptide quantitation 30min gradient (per sample) $120 $192
MRM-LC-MS/MS for peptide quantitation->=60min gradient (per sample) $200 $320
HPLC/MS data interpretation
DescriptionInternal price (Cornell and Cornell affiliates)External price
Quantitation analysis for label-free nanoLC-MS/MS (per set of quan) $250 $400
Customized database interrogation (per database) $75 $120
Additional database search and report (per hour) $100 $160
Additional statistical analysis by Perseus software (per set of data) $300 $480