Sample extraction, clean-up, dilution, re-array
DNA and RNA extraction for plant tissue
Designed for large experiments, we recommend this service if you intend to follow up with our large projects library preparation pipeline. We charged a fixed price corresponding to a 96-well plate, no matter how many samples you actually submit in the plate.
- DNA extraction: Using highly purified and RNA-free columns, our extractions reach an average yield of 2-7 µg of DNA per sample. These extractions are suitable for library preparation for NGS analyses.
- RNA extraction: Using TRIzol, we yield 2-10 µg per sample of total cellular RNA. These extractions are suitable for 3’RNA seq, conventional RNA-seq, or other applications requiring total RNA as the input material.
Our pipelines are optimized for plant DNA/RNA extraction. Occasionally, we perform RNA extraction from non-plant tissues. Please contact us to inquire.
High molecular weight DNA extraction
Regular extractions tend to fragment DNA molecules. For some sequencing technologies, such as DNA sequencing with 10X and MinION, the procedure requires "intact" genomics DNA or high molecular weight DNA (i.e. samples in which the majority of fragments > 40 kb).
We perform extractions using protocols that are designed to minimize DNA breakage. We offer this service for a minimum of 8 samples.
If you choose to prepare your sequencing library with our large projects pipeline, you are responsible for providing samples that have been cleaned after extraction, and at the right concentration. If you need help with these steps, we provide these services using robots that offer a time-saving solution if you have a large number of samples. We use commercial columns or beads (e.g. AMPure XP) and proceed according to manufacturer instructions.
This service is only necessary if you did your DNA/RNA extraction yourself. If we performed the extraction in the facility, we will have already taken the steps to insure the quality of your samples.
Sample concentration or dilution
This service is often added as part of the large projects library preparation pipeline to normalize the concentration of a large batch of samples. Depending on your sample, we can either:
- concentrate your samples using a SpeedVac (for DNA only)
- perform a DNA/RNA cleanup, followed by eluting in a small volume (for DNA or RNA)
If you submit samples that are already normalized for concentration, this step will not be necessary.
There are many reasons for wanting to reorganize samples in a plate. For example, you may want to redistribute and randomize the position of replicates in a plate. Or you may need to select all samples that failed some criteria (e.g. QC), and rearrange them all together in a new plate to be processed. Whatever your reason, we are happy to help with this step, too.
|Description||Internal price (Cornell and Cornell affiliates)||External price|
|DNA extraction (plant leaf tissue only)||$550 per plate**||inquire|
|RNA extraction (limited tissues & species)*||$550 per plate**||inquire|
|DNA cleanup||$300 per plate||inquire|
|Pre- or post-cleanup quantification of DNA or RNA||$400 per plate||inquire|
|DNA/RNA sample dilution||$50 per plate||inquire|
|Sample re-array||$200 per plate||inquire|
|* Pilot study recommended for species or tissues we have not worked with previously.|
** 96 well plate including one blank negative control. A blank negative control (empty well) is required for a full plate of 96 samples.