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Cornell University

Quantitative (comparative) proteomics

Quantitative proteomics (also called comparative proteomics) studies the changes in protein abundance between two different groups or two biological conditions (e.g. samples and controls). The goal is to discover the responsive proteins related to a specific biological perturbation. This is often associated with a pathway analysis to understand the outcome of these changes on a functional level.

The services we offer include:

  • isobaric tag relative absolute protein quantitation (iTRAQ)/Tandem Mass Tag (TMT)
  • stable isotope labeling with amino acids in cell culture (SILAC)
  • dimethyl labeling
  • label-free detection (XIC, spectral counting, and SRM)

For comparative proteomics, most samples should be in solution. In some cases, gel-based samples might be the only option (e.g. some IP-dull down samples) requiring label-free quantitation.

To order a quantitative proteomics analysis, click on the service link below.