10X Genomics single-cell libraries
Our facility is equipped with a Chromium X instrument (10x Genomics) that enables profiling gene expression, cell-surface markers, and/or chromatin accessibility for hundreds to thousands of single cells in parallel. This microfluidic device partitions cells into emulsion droplets where cell-specific barcodes are associated with the cellular readout of interest.
We accept samples submitted as single cell (or single nucleus) suspensions and provide full-service support for all Chromium X assays. Our workflow includes single cell barcoding, library preparation, standard QC steps, and coordinating sequencing. We maintain inventory of the standard throughput 3’ gene expression reagents (and optional Feature Barcoding components) in the Genomics Facility. Our pricing for the 3’ gene expression assay includes the cost of the kits. Although we fully support other Chromium assays, we do not regularly keep stock of the necessary kits for these assays. Therefore, advanced planning and a consultation are necessary for these other assays and their pricing does not reflect the costs of the 10x kits.
We also provide library construction for Visium samples submitted as amplified cDNA.
The listed prices below are **estimates** based on a typical project’s workflow following a single Chromium X run. For most assays, up to 8 samples can be submitted at one time for a single Chromium X run. Pricing will be adjusted on project-by-project basis. Assays or product extensions that involve additional library types (e.g. Feature Barcoding, V(D)J, ATAC), will increase the price based on the number of additional libraries prepared.
The Genomics Facility will coordinate sequencing, which is not included in pricing below.
Sample processing requires a reservation a minimum of 1 week, and ideally 2-3 weeks, in advance.
The listed prices below are estimates based on a typical project’s workflow.
Service pricing
| Description | Internal price (Cornell and Cornell affiliates) | External price |
|---|---|---|
| Single Cell 3'RNA-Seq library – 1 sample, 1 library | $3,170 | $5,205 |
| Single Cell 3'RNA-Seq library - 2 samples, 1 library each | $4,940 | $8,110 |
| Single Cell 3'RNA-Seq library - 3 samples, 1 library each | $6,710 | $11,015 |
| Single Cell 3'RNA-Seq library - 4 samples, 1 library each | $8,840 | $13,920 |
| Single Cell 3'RNA-Seq library - 5 samples, 1 library each | $10,250 | $16,825 |
| Single Cell 3'RNA-Seq library - 6 samples, 1 library each | $12,020 | $19,730 |
| Single Cell 3'RNA-Seq library - 7 samples, 1 library each | $13,790 | $22,635 |
| Single Cell 3'RNA-Seq library - 8 samples, 1 library each | $15,560 | $25,540 |
| Description | Internal price (Cornell and Cornell affiliates) | External price |
|---|---|---|
| Chromium X Assay (Kit Not Included) – 1 sample, 1 library | $1,355 | $2,225 |
| Chromium X Assay (Kit Not Included) – 2 samples, 1 library each | $1,445 | $2,375 |
| Chromium X Assay (Kit Not Included) – 3 samples, 1 library each | $1,535 | $2,525 |
| Chromium X Assay (Kit Not Included) – 4 samples, 1 library each | $1,625 | $2,675 |
| Chromium X Assay (Kit Not Included) – 5 samples, 1 library each | $1,715 | $2,825 |
| Chromium X Assay (Kit Not Included) – 6 samples, 1 library each | $1,805 | $2,975 |
| Chromium X Assay (Kit Not Included) – 7 samples, 1 library each | $1,895 | $3,125 |
| Chromium X Assay (Kit Not Included) – 8 samples, 1 library each | $1,985 | $3,275 |
| Description | Internal price (Cornell and Cornell affiliates) | External price |
|---|---|---|
| Feature Barcoding for 3’RNAseq – 1 sample, 1 library | $655 | $1,075 |
| Feature Barcoding for 3’RNAseq – 2 samples, 1 library each | $860 | $1,410 |
| Feature Barcoding for 3’RNAseq – 3 samples, 1 library each | $1,065 | $1,745 |
| Feature Barcoding for 3’RNAseq – 4 samples, 1 library each | $1,270 | $2,080 |
| Feature Barcoding for 3’RNAseq – 5 samples, 1 library each | $1,475 | $2,415 |
| Feature Barcoding for 3’RNAseq – 6 samples, 1 library each | $1,680 | $2,750 |
| Feature Barcoding for 3’RNAseq – 7 samples, 1 library each | $1,885 | $3,085 |
| Feature Barcoding for 3’RNAseq – 8 samples, 1 library each | $2,090 | $3,420 |
| Description | Internal price (Cornell and Cornell affiliates) | External price |
|---|---|---|
| 10x Library Preparation (kit not included) – 1 sample, 1 library | $525 | $865 |
| 10x Library Preparation (kit not included) – 2 samples, 1 library each | $600 | $990 |
| 10x Library Preparation (kit not included) – 3 samples, 1 library each | $675 | $1,115 |
| 10x Library Preparation (kit not included) – 4 samples, 1 library each | $750 | $1,240 |
| 10x Library Preparation (kit not included) – 5 samples, 1 library each | $825 | $1,365 |
| 10x Library Preparation (kit not included) – 6 samples, 1 library each | $900 | $1,490 |
| 10x Library Preparation (kit not included) – 7 samples, 1 library each | $975 | $1,615 |
| 10x Library Preparation (kit not included) – 8 samples, 1 library each | $1,050 | $1,740 |
| Description | Internal price (Cornell and Cornell affiliates) | External price |
|---|---|---|
| 10x-consultation and analysis | $125 | $205 |