SyproRuby staining
Important: use plastic trays as the dye binds to glass.
- After electrophoresis, fix gel in 40% methanol /50% water/ 10% acetic acid for approximately 1/2 hr.
- Rinse the gel with water at least one time and shake it for another ½ hr.
- Dilute commercial available SyproRuby solution 1:1 with water.
- Expose the gel in this solution at least 18 h overnight and keep it dark.
- Discard the staining solution, rinse the gel with water and place the gel in 10% ethanol/ 90% water for at least 1 hr for background reduction and keep it dark.
- Gels can be stored in the wash solution or in water at 4°C.
| Mini gel (8X10cm) | Standard gel (18x16cm) | |
|---|---|---|
| Volume of fixing and staining solution | 50 ml per gel | 200 ml per gel |