MiSeq

With its ability to produce long paired-end reads (up to 2 x 300 bp), the Illumina MiSeq instrument is ideal for de novo assembly of small genomes or amplicon sequencing projects.

MiSeq have a single lane per flowcell. These instruments can rapidly run a single sample or pool of samples. Currently, the maximum read length is 300 bp. Depending on the application, the run time ranges from 1 to 2 days.

We provide a variety of read length options, as reflected in our price list. With the MiSeq instrument, runs can be configured with some flexibility. For example, a 50 bp kit can be used for a paired-end 2 x 25 bp run. In some cases, we may be able to offer custom read lengths. Please contact us to discuss options for your project.

NextSeq 500/550

The Illumina NextSeq500 instruments are recommended for whole-genome sequencing, exome sequencing, and RNA-Seq experiments. These instruments can process one sample (or pool of samples) on a run.

We provide a variety of read length options, as reflected in our price list. With the Illumina 500/550 instruments, runs can be configured with some flexibility. For example, a 50 bp kit can be used for a paired-end 2 x 25 bp run. In some cases, we may be able to offer custom read lengths. Please contact us to discuss options for your project.

NextSeq 2000

• The NextSeq 2k uses patterned flowcells and Illumina’s ExAmp clustering chemistry, which require more stringent quality control prior to sequencing libraries.
• We also recommend using UDI (unique dual indexing) when prepping samples to be sequenced on this instrument. UDI is a known mitigation for filtering index-hopped reads seen in downstream analyses.

• Illumina’s P2 flowcells produce a similar number of reads when compared with NextSeq 500/550 flowcells, but the smallest kit available for the P2 is 100 bp (vs. 75 bp for the NextSeq 500/550), which may be attractive for certain applications.

• Illumina’s P3 flowcells have sequencing lanes that are physically larger than the P2 flowcells. This allows P3 flowcells to output around 1 billion fragments sequenced, or ~2.5 times the output of a NextSeq 2k or a NextSeq 500/550 flowcell.

We provide a variety of read length options, as reflected in our price list. With the NextSeq 2k, runs can be configured with some flexibility. For example, a 50 bp kit can be used for a paired-end 2 x 25 bp run. In some cases, we may be able to offer custom read lengths. Please contact us to discuss options for your project.