Capabilities
With its ability to produce long paired-end reads (up to 2 x 300 bp), the Illumina MiSeq instrument is ideal for de novo assembly of small genomes or amplicon sequencing projects.
- Runs may be configured with some flexibility (e.g. a 50 bp kit can be used for a paired-end 2 x 25 bp run).
- MiSeq have a single lane per flowcell. These instruments can rapidly run a single sample or pool of samples. Currently, the maximum read length is 300 bp. Depending on the application, the run time ranges from 1 to 2 days.
- The run time and output per flowcell vary depending on the application, as indicated in the tables below.
| MiSeq v2 kits |
Run time |
Reads passing filter (M/lane) |
Pass filter output (Gbp/run) |
| 50 bp kit (single-end 50bp) |
~1 day |
12-15 |
0.6-0.75 |
| 300 bp kit (paired-end 2 x 150 bp) |
~1.5 day |
24-30 |
3.6-4.5 |
| 500 bp kit (paired-end 2 x 250 bp) |
~2 days |
24-30 |
7.2-9 |
| MiSeq v3 kits |
Run time |
Reads passing filter (M/lane) |
Pass filter output (Gbp/run) |
| 150 bp kit (single-end 150bp) |
~1.5 day |
22-25 |
3.3-3.75 |
| 150 bp kit (paired-end 2 x 75 bp) |
~1.5 day |
44-50 |
3.3-3.75 |
| 600 bp kit (paired-end 2 x 300 bp) |
~3 days |
44-50 |
13-15 |
| MiSeq v2 kits |
Run time |
Reads passing filter (M/lane) |
Pass filter output (Gbp/run) |
| 300 bp kit (paired-end 2 x 150 bp) |
inquire |
2 |
0.3 |
| 500 bp kit (paired-end 2 x 350 bp) |
inquire |
2 |
0.5 |
| MiSeq Micro kits |
Run time |
Reads passing filter (M/lane) |
Pass filter output (Gbp/run) |
| 300 bp kit (paired-end 2 x 150 bp) |
inquire |
8 |
1.2 |
Flexibility
We provide a variety of read length options, as reflected in our price list. With the MiSeq instrument, runs can be configured with some flexibility. For example, a 50 bp kit can be used for a paired-end 2 x 25 bp run. In some cases, we may be able to offer custom read lengths. Please contact us to discuss options for your project.
Don't overpay for bad samples
When you place an order for sequencing of a library that you prepared with our Illumina Library small project pipeline, we automatically include an internal QC before sequencing your samples. If the QC results are very poor and sequencing is not recommended, we will not process your samples. You will be charged only for the QC, not the sequencing of samples that are likely to fail.
