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Cornell University

Sony MA900 specifications

The Sony MA900 has a micro fluidic chip-based design and advanced automation that simplifies operation. It offers a high level of automation, aseptic cleaning, QC, and sort setup to ensure optimal daily alignment of the chip to the lasers. Wizard cleaning cycles simplify maintenance. The MA900 software guides operation. A choice of eight default and five custom sort modes is available to achieve the desired purity and yield of the sorted population. Sort devices supported include 2-way and 4-way tubes as well as 6-, 12-, 48, 96-, and 384-well plates. The MA900 is installed in a custom-tailored Class II Type A2 biosafety cabinet for protection of personnel and products. The cabinet incorporates a built-in aerosol management system which operates independently to actively evacuate aerosols from the sort collection chamber. The dual routes of aerosol evacuation maximize protection. Types of sorts include high-purity 4-way sorting of a 12-color immunophenotyping panel and sorting of a 12-color immunophenotyping panel using 5 violet fluorochromes.

Excitation lasers

488 nm, 638 nm, 405nm, 561 nm

Output power

405 nm (10 mW max), 488 nm, 638 nm, and 561 nm (36 mW max)

Detection parameters

12 fluorescence + 2 scatter

Sample tube

Single, auto-loading tube

Tube types

0.5 -mL, 1.5 mL, 5 mL, and 15-mL tubes

Temperature control

5°C, 37°C

Agitation unit

Eccentric rotation

Magnetic drive

300 rpm speed

Sorting chip size (nozzle)

100 um

Sorting speed

100 um (targeted) setting: 21 kHz to 23.5 kHz

100 um (standard) setting: 27 kHz to 31 kHz

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