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Cornell University

Flow Cytometry Facility

Instrument comparison at a glance

Sorters Biosafety Level* Parameters** Lasers (nm) Nozzle size Temperature control (C) Sorting
FACSMelody BSL2* V(3), B(4), R(2) 405, 488, 640 100um 5, 37 2-way | plates + tubes*** (5mL)
Sony MA900 BSL2* V(7), B(5), Y(4), R(3) 405, 488, 561, 640 100um 5, 37 2 and 4-way | plates + tubes (1.5, 2, 5, 15 mL)
FACSAria Fusion BSL2* V(5), B(2), Y(4), R(3) 405, 488, 561, 640 70, 85, 100, and 130um 4, 20, 37, 42 2 and 4-way | plates + tubes (1.5, 2, 5, 15 mL)

 * Biocontainment hood + aerosol management
** B: blue, R: red, UV: ultraviolet, V: violet, Y: yellow

***Polypropylene tubes 

Analyzers Biosafety Level Parameters Lasers (nm) Sampling Tube type* Plate type
FACSymphony A3 BSL1 UV(6), V(6), B(4), Y(4), R(3) 355, 405, 488, 561, 640 Single manually loaded tube 5 mL Currently Unavailable
FACSymphony A5 SE BSL1 UV(10), V(14), B(10), Y(9), R(6) 355, 405, 488, 561, 637 Single manually loaded tube 5 mL Currently Unavailable
Attune NxT BSL1 V(6), B(2), Y(3), R(3) 405, 488, 561, 640 Single manually loaded tube | Plate autosampler 0.5, 1.5, 5 mL 96- and 384-well plates

* The FACSymphony A3 and A5 SE only accept polystyrene tubes

Additional resources

We have a dedicated website for highly specialized and technical information of general interest for the Flow Cytometry community - including publications, video tutorial, protocols, etc.

You can find useful tips and information on the Cornell Center for Immunology’s Flow Cytometry resources page, including panel design do’s and don’ts, data repositories, textbooks, tips, tutorials, protocols and more.

The BRC has two reserved parking spaces for facility customers who are transporting sensitive samples.


The Flow Cytometry Facility is the result of a faculty-led initiative across several colleges on campus. Generous financial support came through the offices of Provost Mike Kotlikoff and Vice Provost for Research Emmanuel Giannelis, the Colleges of Veterinary Medicine, Engineering, Human Ecology, Agriculture and Life Sciences, and Arts and Sciences.

Acknowledging BRC services and staff

If you publish data that has been generated with the help of the services we provide, mentioning the Cornell Institute of Biotechnology in the acknowledgments section is very helpful. Here is why. You can also review our authorship guidelines. Please reference the following Research Resource Identifier (RRID): RRID:SCR_021740