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Flow Cytometry Facility

Instrument comparison at a glance

Sorters Biosafety Level* Parameters** Lasers (nm) Nozzle size Temperature control (C) Sorting
FACSMelody BSL2* V(3), B(4), R(2) 405, 488, 640 100um 5, 37 2-way | plates + tubes*** (5mL)
Sony MA900 BSL2* V(7), B(5), Y(4), R(3) 405, 488, 561, 640 100um 5, 37 2 and 4-way | plates + tubes (1.5, 2, 5, 15 mL)
FACSAria Fusion BSL2* V(5), B(2), Y(4), R(3) 405, 488, 561, 640 70, 85, 100, and 130um 4, 20, 37, 42 2 and 4-way | plates + tubes (1.5, 2, 5, 15 mL)
Biometrica Biosorter BSL1 B(3) 488 500um none 50 mL tubes

 * Biocontainment hood + aerosol management
** B: blue, R: red, UV: ultraviolet, V: violet, Y: yellow

***Polypropylene tubes 

Analyzers Biosafety Level Parameters Lasers (nm) Sampling Tube type* Plate type
FACSymphony BSL1 UV(6), V(6), B(4), Y(4), R(3) 355, 405, 488, 561, 640 Single manually loaded tube 5 mL 96- and 384-well plates
Attune NxT BSL1 V(6), B(2), Y(3), R(3) 405, 488, 561, 640 Single manually loaded tube | Plate autosampler 0.5, 1.5, 5 mL 6- and 384-well plates

* The FACSymphony only accepts polystyrene tubes

Additional resources

You can find useful tips and information on the Cornell Center for Immunology’s Flow Cytometry resources page, including panel design do’s and don’ts, data repositories, textbooks, tips, tutorials, protocols and more.

Support

The Flow Cytometry Facility is the result of a faculty-led initiative across several colleges on campus. Generous financial support came through the offices of Provost Mike Kotlikoff and Vice Provost for Research Emmanuel Giannelis, the Colleges of Veterinary Medicine, Engineering, Human Ecology, Agriculture and Life Sciences, and Arts and Sciences.

Acknowledging BRC services and staff

If you publish data that has been generated with the help of the services we provide, mentioning the Cornell Institute of Biotechnology in the acknowledgments section is very helpful. Here is why. You can also review our authorship guidelines.