Flow Cytometry Facility
Services
Cell Analyzers
Instrument comparison at a glance
| Sorters | Biosafety Level* | Parameters** | Lasers (nm) | Nozzle size | Temperature control (C) | Sorting |
|---|---|---|---|---|---|---|
| FACSMelody | BSL2* | V(3), B(4), R(2) | 405, 488, 640 | 100um | 5, 37 | 2-way | plates + tubes*** (5mL) |
| Sony MA900 | BSL2* | V(7), B(5), Y(4), R(3) | 405, 488, 561, 640 | 100um | 5, 37 | 2 and 4-way | plates + tubes (1.5, 2, 5, 15 mL) |
| FACSAria Fusion | BSL2* | V(5), B(2), Y(4), R(3) | 405, 488, 561, 640 | 70, 85, 100, and 130um | 4, 20, 37, 42 | 2 and 4-way | plates + tubes (1.5, 2, 5, 15 mL) |
* Biocontainment hood + aerosol management
** B: blue, R: red, UV: ultraviolet, V: violet, Y: yellow
***Polypropylene tubes
| Analyzers | Biosafety Level | Parameters | Lasers (nm) | Sampling | Tube type* | Plate type |
|---|---|---|---|---|---|---|
| FACSymphony A3 | BSL1 | UV(6), V(6), B(4), Y(4), R(3) | 355, 405, 488, 561, 640 | Single manually loaded tube | 5 mL | Currently Unavailable |
| FACSymphony A5 SE | BSL1 | UV(10), V(14), B(10), Y(9), R(6) | 355, 405, 488, 561, 637 | Single manually loaded tube | 5 mL | Currently Unavailable |
| Attune NxT | BSL1 | V(6), B(2), Y(3), R(3) | 405, 488, 561, 640 | Single manually loaded tube | Plate autosampler | 0.5, 1.5, 5 mL | 96- and 384-well plates |
* The FACSymphony A3 and A5 SE only accept polystyrene tubes
Additional resources
We have a dedicated website for highly specialized and technical information of general interest for the Flow Cytometry community - including publications, video tutorial, protocols, etc.http://blogs.cornell.edu/brcflow/
You can find useful tips and information on the Cornell Center for Immunology’s Flow Cytometry resources page, including panel design do’s and don’ts, data repositories, textbooks, tips, tutorials, protocols and more.
Support
The Flow Cytometry Facility is the result of a faculty-led initiative across several colleges on campus. Generous financial support came through the offices of Provost Mike Kotlikoff and Vice Provost for Research Emmanuel Giannelis, the Colleges of Veterinary Medicine, Engineering, Human Ecology, Agriculture and Life Sciences, and Arts and Sciences.
Acknowledging BRC services and staff
If you publish data that has been generated with the help of the services we provide, mentioning the Cornell Institute of Biotechnology in the acknowledgments section is very helpful. Here is why. You can also review our authorship guidelines. Please reference the following Research Resource Identifier (RRID): RRID:SCR_021740