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Cornell University

Illumina library: small projects

We construct sequencing libraries from purified DNA, purified total RNA, or purified polyA-selected RNA. For ChIP-seq preps, researchers provide the DNA after the ChIP step.

We prepare the libraries according to standard protocols that yield nearly 100% success rates. All steps are handled manually. In addition, we re-do preparations for samples that fail on the first attempt.

Prep kits for DNA-Seq

  • Nextera Flex (Illumina) — Faster turnover. Very flexible, can be used for sequencing large and complex genomes, microbiome, metagenomics.
  • TruSeq (Illumina) — Can be done PCR-free, or with PCR if the amount of DNA is too low. Offers more coverage and a higher quality than Nextera Flex.
  • TruSeq Nano — We use this kit when there is a very low amount of DNA in the samples. Requires PCR.

Prep kits for RNA-Seq

  • TruSeq (Illumina) — 3' RNA-seq.

Prep kits for ChIP-Seq

  • We do not perform the immunoprecipitation step. The DNA library is created with the commercial TruSeq (Illumina) or TruSeq-equivalent (home-made mix that uses the same ligase as the commercial Illumina kit and gives comparable results).

Service pricing

Illumina library: small projects
DescriptionInternal price (Cornell and Cornell affiliates)External price
TruSeq DNA library prep (Illumina Kit) - with PCR. 1-8 samples* $220 per sample $361 per sample
9-24 samples* $110 per sample $180 per sample
25-96 samples* $99 per sample $162 per sample
PCR-free TruSeq DNA library prep (Illumina kit). 1-8 samples* $193 per sample $316 per sample
9-24 samples* $110 per sample $180 per sample
25-96 samples* $94 per sample $153 per sample
Nextera Flex library prep (Illumina kit). 1-8 samples* $165 per sample $271 per sample
samples 9-24 * $110 per sample $180 per sample
samples 25-96 * $88 per sample $144 per sample
TruSeq RNA, stranded sample library prep (Illumina kit). 1-8 samples* $330 per sample $541 per sample
9-24 samples* $176 per sample $289 per sample
25-96 samples* $138 per sample $226 per sample
* These numbers include one blank well